ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNIAEEPSDE VISSGPEDTD ICSQQTSASA EAGDQSIKIE RKTSTGLQLE QLANTNLLTI 

        70         80         90        100        110        120 
RIKWQLQEEE DDHCNSRITD QIMDTIQHYK GISVNNSDTE TYEFLPDTRR LQVLEQNKDI 

       130        140        150        160        170        180 
YLYEHGSQEY EKSYKDNEEE DDWRYDTVLQ AQFKYPKSLE NACTDISELL KSEPIGQHID 

       190        200        210        220        230        240 
KWSIGVNKHA LTYPGNIFVG GIAKSLSIGE LSFLFSKYGP ILSMKLIYDK TKGEPNGYGF 

       250        260        270        280        290        300 
ISYPLGSQAS LCIKELNGRT VNGSTLFINY HVERKERERI HWDHVKENNN DDNFRCLFIG 

       310        320        330        340        350        360 
NLPYHNPEKV ETLITPKEVI EVIKKELSKK FPDFDIISYY FPKRSNTRSS SSVSFNEEGS 

       370        380        390        400        410        420 
VESNKSSNNT NGNAQDEDML KGYGFIKLIN HEQALAAIET FNGFMWHGNR LVVNKAVQHK 

       430        440        450        460        470        480 
VYNNHNSHDR HPSISNHNDM EVLEFANNPM YDYNNYTYDR YYFNNNKNGN SNDTSNVRYF 

       490        500        510        520        530        540 
DSVRSTPVAE KMDLFYPQRE SFSEGRGQRV PRFMGNKFDM YQYPSTSYSL PIPMSNQQES 

       550        560        570        580        590        600 
NLYVKHIPLS WTDEDLYDFY KSFGEIISVK VITVGGSKNK YRQQSNDSSS DNDLPVGSSR 

       610        620        630        640        650        660 
GYGFVSFESP LDAAKAILNT DGYQVSKDQV LSVSFAQKRG NLSSSDDDDQ SQTDNSSKFQ 

       670        680        690        700        710        720 
NFQPHNDYHK AYPTKYNKKF INALMTQNQS QQQVSRENYF IPLQYPNTNT KPVNSYNLIS 

       730        740        750        760        770        780 
ANQNNANWMM PMFPSFGFIP QVPPVPYIIP PQNPAANHIP IMANGSNEEE EFSSGDYSMD 


Y 

References and documentation are available.

Number of amino acids: 781

Molecular weight: 89512.78

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  28	  3.6%
Arg (R)  26	  3.3%
Asn (N)  75	  9.6%
Asp (D)  50	  6.4%
Cys (C)   5	  0.6%
Gln (Q)  42	  5.4%
Glu (E)  54	  6.9%
Gly (G)  40	  5.1%
His (H)  20	  2.6%
Ile (I)  49	  6.3%
Leu (L)  46	  5.9%
Lys (K)  49	  6.3%
Met (M)  17	  2.2%
Phe (F)  36	  4.6%
Pro (P)  40	  5.1%
Ser (S)  82	 10.5%
Thr (T)  34	  4.4%
Trp (W)   7	  0.9%
Tyr (Y)  45	  5.8%
Val (V)  36	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      3952
Hydrogen    H	      5990
Nitrogen    N	      1072
Oxygen      O	      1268
Sulfur      S	        22

Formula: C3952H5990N1072O1268S22
Total number of atoms: 12304

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   105800
Abs 0.1% (=1 g/l)   1.182, assuming all pairs of Cys residues form cystines


Ext. coefficient   105550
Abs 0.1% (=1 g/l)   1.179, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.21
This classifies the protein as unstable.



Aliphatic index: 64.39

Grand average of hydropathicity (GRAVY): -0.816