ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNIGGGKFLL GRISNNPTSG IVGLANVGKS TFFQAITNSK LGNPANYPFA TIDAECAKVN 

        70         80         90        100        110        120 
IPSVPLSNLL RIYQSAKCVP GTLTIYDIAG LTRGASQGHG LGNKFLNDIR HVEGIFQVVR 

       130        140        150        160        170        180 
GFLKEDITHI EGNVDPVRDL SVVQDELILK DLEFLENIRE RLSKKMRMVS KNSKEHQEMK 

       190        200        210        220        230        240 
IETELLDALE EHLFNGKKIR HFKDHWNLDE VKILNKHNFL TSKPTLILLN VSPQDYVRNE 

       250        260        270        280        290        300 
NKFVRNIIEW INEFSPGDKF LLFSAEFESQ LMECKGIASE YFDKIKEDTN VSDQQLVSAI 

       310        320        330        340        350        360 
PQIILEMRKL LNLISFFTCG PQEVHQWNIR EGTTAQEAAG VIHSDLRETF ISADVIKYDD 

       370        380        390        400 
LKKMEPPLNE SLLKSKGLIK RAGKQYIMQD NDIALFKAAG GKIKK 

References and documentation are available.

Number of amino acids: 405

Molecular weight: 45714.62

Theoretical pI: 7.15

Amino acid composition: 
Ala (A)  21	  5.2%
Arg (R)  16	  4.0%
Asn (N)  28	  6.9%
Asp (D)  22	  5.4%
Cys (C)   4	  1.0%
Gln (Q)  16	  4.0%
Glu (E)  30	  7.4%
Gly (G)  27	  6.7%
His (H)  10	  2.5%
Ile (I)  36	  8.9%
Leu (L)  43	 10.6%
Lys (K)  36	  8.9%
Met (M)   8	  2.0%
Phe (F)  21	  5.2%
Pro (P)  14	  3.5%
Ser (S)  25	  6.2%
Thr (T)  16	  4.0%
Trp (W)   3	  0.7%
Tyr (Y)   7	  1.7%
Val (V)  22	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2049
Hydrogen    H	      3274
Nitrogen    N	       556
Oxygen      O	       602
Sulfur      S	        12

Formula: C2049H3274N556O602S12
Total number of atoms: 6493

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27180
Abs 0.1% (=1 g/l)   0.595, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.589, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.96
This classifies the protein as stable.



Aliphatic index: 97.01

Grand average of hydropathicity (GRAVY): -0.289