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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS VSEFQKKASG

70 80 90 100 110 120
IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK RKIYAIEQSL VQLSQLKKTD

130 140 150 160 170 180
VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM KNISGSFKDV LEERQRLEMA NKDRWQKLTT

190 200 210 220 230 240
DTGHAPADDQ TQSNHAADLT TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML

250 260 270 280 290 300
MEEGQLSNNV YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN

310 320 330 340
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN


References and documentation are available.

Number of amino acids: 340 Molecular weight: 38807.47 Theoretical pI: 6.49
Amino acid composition: 
Ala (A) 20 5.9% Arg (R) 16 4.7% Asn (N) 34 10.0% Asp (D) 16 4.7% Cys (C) 0 0.0% Gln (Q) 30 8.8% Glu (E) 26 7.6% Gly (G) 11 3.2% His (H) 4 1.2% Ile (I) 18 5.3% Leu (L) 31 9.1% Lys (K) 25 7.4% Met (M) 8 2.4% Phe (F) 16 4.7% Pro (P) 6 1.8% Ser (S) 34 10.0% Thr (T) 16 4.7% Trp (W) 3 0.9% Tyr (Y) 5 1.5% Val (V) 21 6.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 42 Total number of positively charged residues (Arg + Lys): 41 Atomic composition: Carbon C 1689 Hydrogen H 2704 Nitrogen N 488 Oxygen O 544 Sulfur S 8 Formula: C1689H2704N488O544S8 Total number of atoms: 5433 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 23950 Abs 0.1% (=1 g/l) 0.617 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 43.86 This classifies the protein as unstable. Aliphatic index: 80.00 Grand average of hydropathicity (GRAVY): -0.682