ProtParam
User-provided sequence:
10 20 30 40 50 60
MNIKDRTSEF QQSVLSYKKR NKNFREQQRE RLQEKESENF ANNTTGNGKS VSEFQKKASG
70 80 90 100 110 120
IAHEISSTAQ LLSKLAVLAK RKPMFNDNPV EIAELSFLIK RKIYAIEQSL VQLSQLKKTD
130 140 150 160 170 180
VNGNTSNQSS KQPSAVQHSK NVVNLLNTQM KNISGSFKDV LEERQRLEMA NKDRWQKLTT
190 200 210 220 230 240
DTGHAPADDQ TQSNHAADLT TYNNSNPFMT SLLDESSEKN NNSSNQGELS FPQNDSQLML
250 260 270 280 290 300
MEEGQLSNNV YLQERNRAVE TIESTIQEVG NLFQQLASMV QEQGEVIQRI DANVDDIDLN
310 320 330 340
ISGAQRELLK YFDRIKSNRW LAAKVFFIIF VFFVIWVLVN
References and
documentation are available.
Number of amino acids: 340
Molecular weight: 38807.47
Theoretical pI: 6.49
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1689
Hydrogen H 2704
Nitrogen N 488
Oxygen O 544
Sulfur S 8
Formula: C
1689H
2704N
488O
544S
8
Total number of atoms: 5433
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 0.617
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.86
This classifies the protein as unstable.
Aliphatic index: 80.00
Grand average of hydropathicity (GRAVY): -0.682