ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNILKHFPSY VGPSKIRTLV IPIGHWTRKE FNNAVQKLSE FNEIHLSDVT PIDSPIFTPQ 

        70         80         90        100        110        120 
GFPHGKLFFD FLTIDHDDAL ELFLYDFEPF RKTFVIIGLV NDYSDPLTNL NFMKEKYPTL 

       130        140        150        160        170        180 
ISPNLVYASS TPTKELEQTI DTMENVFASS PDMQKNIETI MCDIARNFLT ALNSYYSSYK 

       190        200        210        220        230        240 
HVTLRSPGAI GGNAVLKTTL IRQNSYTSSS SSTPMSAVQS SVSSSSKAGS VTTASKRLSS 

       250        260        270        280        290        300 
FEMTTNSLKR SASLKLATTL STSENRSQQK SLGRQMKILG NFQLLAGRYV DALNSFVDAI 

       310        320        330        340        350        360 
TTLYKVRDYL WLGSALDGIS ICFLLLSYLG LSYQIPQIVS LICPVEKLNF ESSSTGISPV 

       370        380        390        400        410        420 
DSNSKATAST TASSTPRNSI SIAAMQSPRN SIMSLSAPAL NIDVENINLP LLIKCISDKV 

       430        440        450        460        470        480 
LYYYDLSLMH NSEYAPQVVY CEFLLKTLTF MTSCYKSSEF SKDVLDNIVK NQHRALSDIP 

       490        500        510        520        530        540 
NSPMFPRFEV YFYSNKLFEL QLKEMQVEAQ IKIYSTMAEV YRLLGYKRKQ LFVLRLLMVA 

       550        560        570        580        590        600 
LLATPNKIAW HPDYRTLIDT IIELLNINES EAKINVDDPS QSTWLILQKK ILQLCIKVSR 

       610        620        630        640        650        660 
KINDFEYVAK FSSILITKYT HLLNQSEQDA LFKEYIQPSI TNESITSYWD PFILREVVIN 

       670        680        690        700        710        720 
RILDSDPTSN EIPLESDVSS LESLENRQKT QDINPQEVFN PFKRVQPTSF VSNNSTKVPI 

       730        740        750        760        770        780 
LVFLVGDKAE FTCRVQNPFK FDFTINDIQL DEEISEFCEI DRKAVSYSGP YNVKAESIRS 

       790        800        810        820        830        840 
ITLPLIIKKP TYKKIYEISC LKISILKLPL QKFDIINDSR RSNPVEEEAE YSKCIYGKLK 

       850        860        870        880        890        900 
IKILPEQPQL ELLSTSKMTR NSWMMLDGTK TDFHITVRNK SLSCAINHIK IIPMNNIEQM 

       910        920        930        940        950        960 
LKPDYWKKMP PDDLYIMEKQ LDWLSKSCVR IIKLPTVIKP NETITFDLEL DNTAVPFNFT 

       970        980        990       1000       1010       1020 
GFDLLIEYGM SATDESCIYL KKLSIPYEVT LRRTIEVPSM DIIPLNELFS SQVENVDWIE 

      1030       1040       1050       1060       1070       1080 
YVMSKIRAES NLHSRDFILL LLDFRNSWID GIKLNVQFED FTSNEYHVEA SHTSRIIVPI 

      1090       1100       1110       1120       1130       1140 
KKIDYKKYNF ENTPIPRIYP GRQFIQSGLN EEQTIEMRQK FWCREHIISK LKCNWKLTTD 

      1150       1160       1170       1180       1190       1200 
QSVTGSVDFN KFIEKFDHKM VYTIYPGRLF YGVQLLLDEP KVKVGEIINL KIITEPTSTC 

      1210       1220       1230       1240       1250       1260 
RRKQNSTVNF LDIVIFDSKT SKILPRSNRR ILYNGSLTKP ISTTKVSEIN LEIIPIEKGR 

      1270       1280 
YEFSVCISKS NNQDGIIQFD SENVILSVI 

References and documentation are available.

Number of amino acids: 1289

Molecular weight: 147662.14

Theoretical pI: 7.45

Amino acid composition: 
Ala (A)  43	  3.3%
Arg (R)  50	  3.9%
Asn (N)  77	  6.0%
Asp (D)  66	  5.1%
Cys (C)  18	  1.4%
Gln (Q)  47	  3.6%
Glu (E)  78	  6.1%
Gly (G)  33	  2.6%
His (H)  17	  1.3%
Ile (I) 126	  9.8%
Leu (L) 131	 10.2%
Lys (K)  95	  7.4%
Met (M)  28	  2.2%
Phe (F)  63	  4.9%
Pro (P)  65	  5.0%
Ser (S) 137	 10.6%
Thr (T)  82	  6.4%
Trp (W)  12	  0.9%
Tyr (Y)  51	  4.0%
Val (V)  70	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 144
Total number of positively charged residues (Arg + Lys): 145

Atomic composition:

Carbon      C	      6672
Hydrogen    H	     10548
Nitrogen    N	      1704
Oxygen      O	      1972
Sulfur      S	        46

Formula: C6672H10548N1704O1972S46
Total number of atoms: 20942

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   143115
Abs 0.1% (=1 g/l)   0.969, assuming all pairs of Cys residues form cystines


Ext. coefficient   141990
Abs 0.1% (=1 g/l)   0.962, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.33
This classifies the protein as unstable.



Aliphatic index: 96.84

Grand average of hydropathicity (GRAVY): -0.185