ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNIVKLQRKF PILTQEDLFS TIEKFRAIDL DDKGWVEKQQ ALEAVSKDGD ATYDEARETL 

        70         80         90        100        110        120 
KHVGVDASGR VELDDYVGLV AKLRESKTGA APQTTFNVAP NSTPIVSTAA TGLQHKGKGT 

       130        140        150        160        170        180 
QAKIIVAGSQ TGTTHTINEE ERREFTKHIN SVLAGDQDIG DLLPFPTDTF QLFDECRDGL 

       190        200        210        220        230        240 
VLSKLINDSV PDTIDTRVLN WPKKGKELNN FQASENANIV INSAKAIGCV VVNVHSEDII 

       250        260        270        280        290        300 
EGREHLILGL IWQIIRRGLL SKIDIKLHPE LYRLLEDDET LEQFLRLPPE QILLRWFNYH 

       310        320        330        340        350        360 
LKQANWNRRV TNFSKDVSDG ENYTILLNQL DPALCSKAPL QTTDLMERAE QVLQNAEKLD 

       370        380        390        400        410        420 
CRKYLTPSSL VAGNPKLNLA FVAHLFNTHP GLEPIQEEEK PEIEEFDAEG EREARVFTLW 

       430        440        450        460        470        480 
LNSLDVDPPV ISLFDDLKDG LILLQAYEKV MPGAVDFKHV NKRPASGAEI SRFKALENTN 

       490        500        510        520        530        540 
YAVDLGRAKG FSLVGIEGSD IVDGNKLLTL GLVWQLMRRN ISITMKTLSS SGRDMSDSQI 

       550        560        570        580        590        600 
LKWAQDQVTK GGKNSTIRSF KDQALSNAHF LLDVLNGIAP GYVDYDLVTP GNTEEERYAN 

       610        620        630        640 
ARLAISIARK LGALIWLVPE DINEVRARLI ITFIASLMTL NK 

References and documentation are available.

Number of amino acids: 642

Molecular weight: 71772.72

Theoretical pI: 5.30

Amino acid composition: 
Ala (A)  47	  7.3%
Arg (R)  33	  5.1%
Asn (N)  36	  5.6%
Asp (D)  46	  7.2%
Cys (C)   4	  0.6%
Gln (Q)  26	  4.0%
Glu (E)  46	  7.2%
Gly (G)  39	  6.1%
His (H)  12	  1.9%
Ile (I)  44	  6.9%
Leu (L)  78	 12.1%
Lys (K)  41	  6.4%
Met (M)   7	  1.1%
Phe (F)  23	  3.6%
Pro (P)  25	  3.9%
Ser (S)  36	  5.6%
Thr (T)  38	  5.9%
Trp (W)   9	  1.4%
Tyr (Y)  11	  1.7%
Val (V)  41	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      3197
Hydrogen    H	      5113
Nitrogen    N	       877
Oxygen      O	       974
Sulfur      S	        11

Formula: C3197H5113N877O974S11
Total number of atoms: 10172

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66140
Abs 0.1% (=1 g/l)   0.922, assuming all pairs of Cys residues form cystines


Ext. coefficient    65890
Abs 0.1% (=1 g/l)   0.918, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.28
This classifies the protein as stable.



Aliphatic index: 99.95

Grand average of hydropathicity (GRAVY): -0.281