ProtParam
User-provided sequence:
10 20 30 40 50 60
MNIYIFSKEK KRKKVVCDNL SKTRGSRREH CEPHRRRMGQ STSKFRRSKT TFTSPVLPNL
70 80 90 100 110 120
REQNSGADEP YDYISNLPDE CLSLIFQSLT CADLKRCSLV CRRWLTIEGQ CRHRLSLKAQ
130 140 150 160 170 180
SDLISVIPSL FTRFDSVTKL VLRSDRRSLG ICDNAFVMIS VRCRNLTRLK LRGCPEISDL
190 200 210 220 230 240
GIIGFTENCR SLKKVSFGSC GFGVKGMNAL LNTCLGLEEL SVKRLRGIGA GAELIGPGGA
250 260 270 280 290 300
AGSLKVICLK ELHNGQCFAP LLSGAKGLRI LKIFRCSGDW DRVFEAVRDK VNAIVEIHLE
310 320 330 340 350 360
RIQMSDLGLT ALSKCSGVEV LHLVKTPDCT NVGLALVAER CKLLRKLHID GWKTNRIGDE
370 380 390 400 410 420
GLIVVAKYCW NLQELVLIGV NPTKLSLEAI VSNCLNLERL ALCGSDTVGD TELCCIAEKC
430 440 450 460 470 480
LALRKLCIKN CPITDDGIKA LGNGCPNLLK VKVKKCRGVT TQGADLLRKR RALLVVNLDA
490 500 510 520 530 540
PETPIVEGSV GEGGAQENAV EFPPSRLQIP TIGLASGSTS RSSSFKLRLG FLSQRNFVSC
550
ALRRLGSRSR SRNE
References and
documentation are available.
Number of amino acids: 554
Molecular weight: 61005.36
Theoretical pI: 9.50
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 2657
Hydrogen H 4430
Nitrogen N 794
Oxygen O 774
Sulfur S 35
Formula: C
2657H
4430N
794O
774S
35
Total number of atoms: 8690
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29835
Abs 0.1% (=1 g/l) 0.489, assuming all pairs of Cys residues form cystines
Ext. coefficient 27960
Abs 0.1% (=1 g/l) 0.458, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.06
This classifies the protein as unstable.
Aliphatic index: 100.63
Grand average of hydropathicity (GRAVY): -0.081