ProtParam
User-provided sequence:
10 20 30 40 50 60
MNIYTSPTRT PNIAPKSGQR PSLPMLATDE RSTDKESPNE DREFVPCSSL DVRRIYPKGP
70 80 90 100 110 120
LLVLPEKIYL YSEPTVKELL PFDVVINVAE EANDLRMQVP AVEYHHYRWE HDSQIALDLP
130 140 150 160 170 180
SLTSIIHAAT TKREKILIHC QCGLSRSATL IIAYIMKYHN LSLRHSYDLL KSRADKINPS
190 200
IGLIFQLMEW EVALNAKTNV QANSYRKVP
References and
documentation are available.
Number of amino acids: 209
Molecular weight: 23885.50
Theoretical pI: 7.74
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1067
Hydrogen H 1702
Nitrogen N 292
Oxygen O 313
Sulfur S 8
Formula: C
1067H
1702N
292O
313S
8
Total number of atoms: 3382
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26025
Abs 0.1% (=1 g/l) 1.090, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.084, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.51
This classifies the protein as unstable.
Aliphatic index: 96.12
Grand average of hydropathicity (GRAVY): -0.342