ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNKEELLGFL LDDSIDSQKR CVTDHEAYSN WLKNDNDERT AHEESSSQST IAALNKKKQT 

        70         80         90        100        110        120 
EAAQEDIEEL LNGLEGIIGG ADPRNLKSKS KRKTKKGGSK PREENVNTEK HIVMLEVEDF 

       130        140        150        160        170        180 
SDMSTHEDVN GASPSPNLDR SKKNEKRRKN AKELSYDELK DKLEVTTRKS RLECKDLKKK 

       190        200        210        220        230        240 
VHGLERRNLE LEQRLEELKI ENQTLIEINN KLLKNTNEDE INKSQRNKEK DRKRRERRTA 

       250        260        270 
RRKDERKQEK KQEKKQDNKT SQSFPSSTDM NGQPIEF 

References and documentation are available.

Number of amino acids: 277

Molecular weight: 32303.00

Theoretical pI: 8.90

Amino acid composition: 
Ala (A)  10	  3.6%
Arg (R)  22	  7.9%
Asn (N)  23	  8.3%
Asp (D)  20	  7.2%
Cys (C)   2	  0.7%
Gln (Q)  12	  4.3%
Glu (E)  37	 13.4%
Gly (G)  10	  3.6%
His (H)   5	  1.8%
Ile (I)  11	  4.0%
Leu (L)  25	  9.0%
Lys (K)  40	 14.4%
Met (M)   4	  1.4%
Phe (F)   4	  1.4%
Pro (P)   6	  2.2%
Ser (S)  22	  7.9%
Thr (T)  14	  5.1%
Trp (W)   1	  0.4%
Tyr (Y)   2	  0.7%
Val (V)   7	  2.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      1363
Hydrogen    H	      2273
Nitrogen    N	       429
Oxygen      O	       465
Sulfur      S	         6

Formula: C1363H2273N429O465S6
Total number of atoms: 4536

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8605
Abs 0.1% (=1 g/l)   0.266, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.263, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 69.64
This classifies the protein as unstable.



Aliphatic index: 61.62

Grand average of hydropathicity (GRAVY): -1.523