ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNKEEQEDPQ QEQISTVQEN DPRNLQQLGM LLVSPGLDED RLSEKMISKI KKSRDIEKNQ 

        70         80         90        100        110        120 
KLLISRLSQK EEDHSGKPPT ITTSPAEKTV PFKSLNHSLK RKRVPPALNF SDIQASSHLH 

       130        140        150        160        170        180 
GSKSAPPNIT RFPQHKNSLR VRYMGRMAPT NQDYHPSVAN SYMTATYPYP YTGLPPVPCY 

       190        200        210        220        230        240 
PYSSTPTQTH AYEGYYSPMY PGPLYNNGII PADYHAKRKK LAGRSPHLED LTSRKRTFVS 

       250        260        270        280        290        300 
KHHNGDPIIS KTDEDIECSV TKNSLSEGAS LNDDADDDND KERIIIGEIS LYDDVFKFEV 

       310        320 
RDDKNDYMKA CETIWTEWHN LKK 

References and documentation are available.

Number of amino acids: 323

Molecular weight: 36879.31

Theoretical pI: 6.87

Amino acid composition: 
Ala (A)  14	  4.3%
Arg (R)  16	  5.0%
Asn (N)  18	  5.6%
Asp (D)  24	  7.4%
Cys (C)   3	  0.9%
Gln (Q)  13	  4.0%
Glu (E)  21	  6.5%
Gly (G)  13	  4.0%
His (H)  12	  3.7%
Ile (I)  18	  5.6%
Leu (L)  23	  7.1%
Lys (K)  28	  8.7%
Met (M)   8	  2.5%
Phe (F)   6	  1.9%
Pro (P)  27	  8.4%
Ser (S)  31	  9.6%
Thr (T)  19	  5.9%
Trp (W)   2	  0.6%
Tyr (Y)  16	  5.0%
Val (V)  11	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1616
Hydrogen    H	      2534
Nitrogen    N	       456
Oxygen      O	       511
Sulfur      S	        11

Formula: C1616H2534N456O511S11
Total number of atoms: 5128

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34965
Abs 0.1% (=1 g/l)   0.948, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.945, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.97
This classifies the protein as unstable.



Aliphatic index: 63.72

Grand average of hydropathicity (GRAVY): -0.976