ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNKESKDDDM SLGKFSFSHF LYYLVLIVVI VYGLYKLFTG HGSDINFGKF LLRTSPYMWA 

        70         80         90        100        110        120 
NLGIALCVGL SVVGAAWGIF ITGSSMIGAG VRAPRITTKN LISIIFCEVV AIYGLIIAIV 

       130        140        150        160        170        180 
FSSKLTVATA ENMYSKSNLY TGYSLFWAGI TVGASNLICG IAVGITGATA AISDAADSAL 

       190        200        210 
FVKILVIEIF GSILGLLGLI VGLLMAGKAS EFQ 

References and documentation are available.

Number of amino acids: 213

Molecular weight: 22595.74

Theoretical pI: 8.39

Amino acid composition: 
Ala (A)  21	  9.9%
Arg (R)   3	  1.4%
Asn (N)   7	  3.3%
Asp (D)   6	  2.8%
Cys (C)   3	  1.4%
Gln (Q)   1	  0.5%
Glu (E)   5	  2.3%
Gly (G)  24	 11.3%
His (H)   2	  0.9%
Ile (I)  25	 11.7%
Leu (L)  25	 11.7%
Lys (K)  10	  4.7%
Met (M)   6	  2.8%
Phe (F)  13	  6.1%
Pro (P)   2	  0.9%
Ser (S)  20	  9.4%
Thr (T)  11	  5.2%
Trp (W)   3	  1.4%
Tyr (Y)   9	  4.2%
Val (V)  17	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	      1052
Hydrogen    H	      1655
Nitrogen    N	       247
Oxygen      O	       284
Sulfur      S	         9

Formula: C1052H1655N247O284S9
Total number of atoms: 3247

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30035
Abs 0.1% (=1 g/l)   1.329, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.324, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 12.70
This classifies the protein as stable.



Aliphatic index: 124.55

Grand average of hydropathicity (GRAVY): 0.919