ProtParam
User-provided sequence:
10 20 30 40 50 60
MNKESKDDDM SLGKFSFSHF LYYLVLIVVI VYGLYKLFTG HGSDINFGKF LLRTSPYMWA
70 80 90 100 110 120
NLGIALCVGL SVVGAAWGIF ITGSSMIGAG VRAPRITTKN LISIIFCEVV AIYGLIIAIV
130 140 150 160 170 180
FSSKLTVATA ENMYSKSNLY TGYSLFWAGI TVGASNLICG IAVGITGATA AISDAADSAL
190 200 210
FVKILVIEIF GSILGLLGLI VGLLMAGKAS EFQ
References and
documentation are available.
Number of amino acids: 213
Molecular weight: 22595.74
Theoretical pI: 8.39
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 1052
Hydrogen H 1655
Nitrogen N 247
Oxygen O 284
Sulfur S 9
Formula: C
1052H
1655N
247O
284S
9
Total number of atoms: 3247
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30035
Abs 0.1% (=1 g/l) 1.329, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 1.324, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 12.70
This classifies the protein as stable.
Aliphatic index: 124.55
Grand average of hydropathicity (GRAVY): 0.919