ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNKTNWKVSV TTFNCGKEFP VENSKAIVKQ LLFPYDDGIS QLELQDLYVL GFQEVVPIWQ 

        70         80         90        100        110        120 
GSFPAVNRDL IDRITTTAVN CLNEKVSATQ GDEQYSCLGV NSLGAITIIV LYNNNALKVK 

       130        140        150        160        170        180 
DDILKRNGKC GWFGTHLKGG TLISFQMTRN GEENWERFSY ICAHLNANEG VNNRNQRIDD 

       190        200        210        220        230        240 
YKRIMSEVCD SEVAKSDHFF FLGDLNFRVT STYDPTTNYS STTTLRRLLE NHEELNLLRK 

       250        260        270        280        290        300 
GEDEPLCKGF QELKITFPPT YKFKLFEKET YNTKRIPSWC DRILYKSYAV PTFAQEGTYH 

       310        320        330        340        350        360 
SVPRSNALLF SDHQPVNLTV RLPRSTGTPV PLSLHIEKYP LSWSSGLIGQ IGDAVIGYCG 

       370        380 
WLVTKNVHYW ILGSLLLYLL LKIL 

References and documentation are available.

Number of amino acids: 384

Molecular weight: 43799.01

Theoretical pI: 7.55

Amino acid composition: 
Ala (A)  13	  3.4%
Arg (R)  17	  4.4%
Asn (N)  27	  7.0%
Asp (D)  18	  4.7%
Cys (C)   9	  2.3%
Gln (Q)  13	  3.4%
Glu (E)  22	  5.7%
Gly (G)  25	  6.5%
His (H)   8	  2.1%
Ile (I)  22	  5.7%
Leu (L)  45	 11.7%
Lys (K)  24	  6.2%
Met (M)   3	  0.8%
Phe (F)  18	  4.7%
Pro (P)  16	  4.2%
Ser (S)  26	  6.8%
Thr (T)  28	  7.3%
Trp (W)   8	  2.1%
Tyr (Y)  17	  4.4%
Val (V)  25	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1980
Hydrogen    H	      3067
Nitrogen    N	       523
Oxygen      O	       576
Sulfur      S	        12

Formula: C1980H3067N523O576S12
Total number of atoms: 6158

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69830
Abs 0.1% (=1 g/l)   1.594, assuming all pairs of Cys residues form cystines


Ext. coefficient    69330
Abs 0.1% (=1 g/l)   1.583, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.27
This classifies the protein as stable.



Aliphatic index: 90.31

Grand average of hydropathicity (GRAVY): -0.271