ProtParam
User-provided sequence:
10 20 30 40 50 60
MNLAGSTIQA YRQRKIESLA KMSNMSQDDV TTGLRTVQQG LEALREEHST ISNTLETSVK
70 80 90 100 110 120
GVKEDEAPLP KQKLSQINDN LDKLVCGVDE TSLMLMVFQL TQGMDAQHQK YQAQRRRLCQ
130 140 150 160 170 180
ENAWLRDELS STQIKLQQSE QMVAQLEEEN KHLKYMASIK QFDDGTQSDT KTSVDVGPQP
190 200 210 220 230 240
VTNETLQELG FGPEDEEDMN ASQFNQPTPA NQMAASANVG YEIPARLRTL HNLVIQYASQ
250 260 270 280 290 300
GRYEVAVPLC KQALEDLEKT SGHDHPDVAT MLNILALVYR DQNKYKEAAN LLNEALSIRE
310 320 330 340 350 360
KCLGESHPAV AATLNNLAVL FGKRGKFKDA EPLCKRALEI REKVLGDDHP DVAKQLNNLA
370 380 390 400 410 420
LLCQNQGKYE EVEKYYKRAL EIYESKLGPD DPNVAKTKNN LSSAYLKQGK YKEAEELYKQ
430 440 450 460 470 480
ILTRAHEREF GQISGENKPI WQIAEEREEN KHKGEGATAN EQAGWAKAAK VDSPTVTTTL
490 500 510 520
KNLGALYRRQ GKYEAAETLE DVALRAKKQM SESRRMERSV MY
References and
documentation are available.
Number of amino acids: 522
Molecular weight: 58825.26
Theoretical pI: 5.77
Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 2546
Hydrogen H 4109
Nitrogen N 735
Oxygen O 823
Sulfur S 20
Formula: C
2546H
4109N
735O
823S
20
Total number of atoms: 8233
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43695
Abs 0.1% (=1 g/l) 0.743, assuming all pairs of Cys residues form cystines
Ext. coefficient 43320
Abs 0.1% (=1 g/l) 0.736, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.09
This classifies the protein as unstable.
Aliphatic index: 77.43
Grand average of hydropathicity (GRAVY): -0.755