ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLAVASFRG NFGVLSQCSS CCSLQFQPFV AATSSLNFGQ TGTSRRKKDL KLERVFRKRE 

        70         80         90        100        110        120 
TLVRVTETQT EPEGNDDEDN KEGKESSADD PPTKIPTELN SQSTVVNEAP GNEEENKAQF 

       130        140        150        160        170        180 
SSQDGDKLEV SSGSPLPGVN VSIIIHVIYK DDSIMFSGCL SFIKSCCEQP LQLDDSMRLP 

       190        200        210        220        230        240 
KETIDILRGQ VFGFDTFFVT SQEPYEGGVL FKGNLRGKPA TSYEKIKTRM ENNFGDQYKL 

       250        260        270        280        290        300 
FLLTNPEDDK PVAVVVPRRS LEPETTAVPE WFAAGSFGLV ALFTLFLRNV PALQSDLLSA 

       310        320        330        340        350        360 
FDNLELLKDG LPGALVTALV LGVHELGHIL VANSLGIKLG VPFFVPSWQI GSFGAITRIK 

       370        380        390        400        410        420 
NIVAKREDLL KVAAAGPLAG FSLGLILFLI GLFVPPSDGI GVVVDASVFH ESFLAGGIAK 

       430        440        450        460        470        480 
LLLGDALKEG TSISLNPLVI WAWAGLLING INSIPAGELD GGKIAFSIWG RKTATRLTGA 

       490        500        510        520        530        540 
SIALLGLSAL FSDVAFYWVV LIFFLQRGPI APLAEEITVP DDKYVSLGIL VLFLSLLVCL 

       550 
PYPFAFTGNE AMMIGL 

References and documentation are available.

Number of amino acids: 556

Molecular weight: 60064.26

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  41	  7.4%
Arg (R)  19	  3.4%
Asn (N)  22	  4.0%
Asp (D)  28	  5.0%
Cys (C)   7	  1.3%
Gln (Q)  16	  2.9%
Glu (E)  33	  5.9%
Gly (G)  50	  9.0%
His (H)   4	  0.7%
Ile (I)  33	  5.9%
Leu (L)  73	 13.1%
Lys (K)  28	  5.0%
Met (M)   6	  1.1%
Phe (F)  37	  6.7%
Pro (P)  31	  5.6%
Ser (S)  45	  8.1%
Thr (T)  27	  4.9%
Trp (W)   6	  1.1%
Tyr (Y)   7	  1.3%
Val (V)  43	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      2736
Hydrogen    H	      4309
Nitrogen    N	       693
Oxygen      O	       796
Sulfur      S	        13

Formula: C2736H4309N693O796S13
Total number of atoms: 8547

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43805
Abs 0.1% (=1 g/l)   0.729, assuming all pairs of Cys residues form cystines


Ext. coefficient    43430
Abs 0.1% (=1 g/l)   0.723, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.95
This classifies the protein as stable.



Aliphatic index: 104.15

Grand average of hydropathicity (GRAVY): 0.215