ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLFWPSETK KQNEIPGGDY TPGNSPSVQK GYQFLNRDIF KSCPRIMERQ FGECLHNRTH 

        70         80         90        100        110        120 
LIKDLISSGN VGLGPIEIVH MSYLNKHEKE EFGEYFYVTG IEVSGPAMPV EFLEVLKSSK 

       130        140        150        160        170        180 
RISKNISNNI ILTYCCFNFF SNLDIRIRYD ADDTFQTTAI DCNKETTDLT MTEKMWEETF 

       190        200        210        220        230        240 
ASSVIRAIIT NTNPELKPPG LVECPFYVGK DTISSCKKII ELLCRFLPRS LNCGWDSTKS 

       250        260        270        280        290        300 
MQATIVNNYL MYSLKSFIAI TPSLVDFTID YLKGLTKKDP IHDIYYKTAM ITILDHIETK 

       310        320        330        340        350        360 
ELDMITILNE TLDPLLSLLN DLPPRDADSA RLMNCMSDLL NIQTNFLLNR GDYELALGVS 

       370        380        390        400        410        420 
NTSTELALDS FESWYNLARC HIKKEEYEKA LFAINSMPRL RKNDGHLETM YSRFLTSNYY 

       430        440        450        460        470        480 
KKPLNGTREH YDLTAMEFTN LSGTLRNWKE DELKRQIFGR IAMINEKKIG YTKEIWDDIA 

       490        500        510        520        530        540 
IKLGPICGPQ SVNLINYVSP QEVKNIKNIN LIARNTIGKQ LGWFSGKIYG LLMEIVNKIG 

       550        560        570        580        590        600 
WNGLLNIRTE AFMMETEFYQ ASNNIIDENG HIPMESRKKR FCEGWLDDLF LDLYQDLKLS 

       610        620        630        640        650        660 
KISLSNKDEK HSGLEWELLG LIMLRTWHWE DAVACLRTSI VARFDPVSCQ QLLKIYLQPP 

       670        680        690        700        710        720 
KNIQEVTLLD TDTIISLLIK KISYDCRYYN YCQIFNLQLL EKLCNELGTH ILRNKILLQP 

       730        740 
SIGDEIMVMI DAMLAWIADL DHTVQP 

References and documentation are available.

Number of amino acids: 746

Molecular weight: 86042.31

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  27	  3.6%
Arg (R)  30	  4.0%
Asn (N)  51	  6.8%
Asp (D)  44	  5.9%
Cys (C)  18	  2.4%
Gln (Q)  21	  2.8%
Glu (E)  51	  6.8%
Gly (G)  36	  4.8%
His (H)  14	  1.9%
Ile (I)  71	  9.5%
Leu (L)  89	 11.9%
Lys (K)  52	  7.0%
Met (M)  24	  3.2%
Phe (F)  29	  3.9%
Pro (P)  29	  3.9%
Ser (S)  48	  6.4%
Thr (T)  46	  6.2%
Trp (W)  13	  1.7%
Tyr (Y)  30	  4.0%
Val (V)  23	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      3865
Hydrogen    H	      6077
Nitrogen    N	      1001
Oxygen      O	      1133
Sulfur      S	        42

Formula: C3865H6077N1001O1133S42
Total number of atoms: 12118

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117325
Abs 0.1% (=1 g/l)   1.364, assuming all pairs of Cys residues form cystines


Ext. coefficient   116200
Abs 0.1% (=1 g/l)   1.350, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.29
This classifies the protein as unstable.



Aliphatic index: 96.21

Grand average of hydropathicity (GRAVY): -0.234