ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLLDGKKCA ESLVAEIARK VAGYTESGLR TPHMTIILVG EHAPSESYVK SKIATSGLAG 

        70         80         90        100        110        120 
FESTLLRFPE TIRESELLAK IKEVNEDPTT DGLIVQLPLP KHINQQHVIN AIAPEKDIDG 

       130        140        150        160        170        180 
FHPANFGRMT LGQKAFRPAT AYGICKLLQF YEVPVKAKHC VVIGRSNIVG KPISIMLSND 

       190        200        210        220        230        240 
FDIGNATVTL THIETPRELL LDETRRADIV IVAVGIPGFV TADMVKDGVV LIDVGINRLE 

       250        260        270        280        290 
SGKIVGDVDF ENVAQKCSWI TPVPGGVGRM TVAALMINTL MAYQNNFNLA 

References and documentation are available.

Number of amino acids: 290

Molecular weight: 31485.57

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  24	  8.3%
Arg (R)  12	  4.1%
Asn (N)  14	  4.8%
Asp (D)  14	  4.8%
Cys (C)   4	  1.4%
Gln (Q)   7	  2.4%
Glu (E)  18	  6.2%
Gly (G)  23	  7.9%
His (H)   7	  2.4%
Ile (I)  26	  9.0%
Leu (L)  26	  9.0%
Lys (K)  17	  5.9%
Met (M)   8	  2.8%
Phe (F)  10	  3.4%
Pro (P)  15	  5.2%
Ser (S)  13	  4.5%
Thr (T)  19	  6.6%
Trp (W)   1	  0.3%
Tyr (Y)   5	  1.7%
Val (V)  27	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1406
Hydrogen    H	      2279
Nitrogen    N	       379
Oxygen      O	       413
Sulfur      S	        12

Formula: C1406H2279N379O413S12
Total number of atoms: 4489

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13200
Abs 0.1% (=1 g/l)   0.419, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.411, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.52
This classifies the protein as stable.



Aliphatic index: 105.21

Grand average of hydropathicity (GRAVY): 0.114