ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLLIDRMEN PGSRNCTLLP PSFPRGFCKG RRASSGDAVK IKESGLQPQP QPEPLQAKTN 

        70         80         90        100        110        120 
VAHFSKSSSR LPVIAVNDNP VVPRPSTEVN LGSLLQKERE KEKEEQPALH DRRHLYVTKN 

       130        140        150        160        170        180 
RAHGVRQRSL EMTSLPVLGS TKTGKFSDFL FEDDIDNRVG RHSRSYSGAS SLDDPFRVSP 

       190        200        210        220        230        240 
KTDFNSNRAR LSCLSKGRRG SMSVFQSCHT GLAFNQIQGS SSSQRRSSAG SFDYERKRLV 

       250        260        270        280        290        300 
NQFLQPSLGN SDPFDTLRES VVFEPSSTAG GIKLGNMHSQ SQISVNSSPS TSLFYHDLDG 

       310        320        330        340        350        360 
SAVNDSSSFL YSRSNVPAFL SSSAFSSTSS TSSDSEDVDR RSLNGVYPSL GYLTNQRKPR 

       370        380        390        400        410        420 
NSSGSSTAPG TDTLGFKYLL NRQKSADSST RFKSVLKVNN NNGSAATPDS SSNSISKSNS 

       430        440        450        460        470        480 
NLNDNIDELN YYQNHISTLL VKIENEMRRN LNDTIIKNEN NVQKTIQKYD LLSGELTLLL 

       490        500        510        520        530        540 
DEMTTLRTTV INQFLVKLKS DFDEDDNKAF INELKISVEE SVAQLQGLER RMEVCQERLN 

       550        560        570        580        590        600 
KQKSSLREMD SLIELKNVLN KSKNNTKSIY LYRYFIIDII AFLLMGGFIV YVKNLLTRFF 


TR 

References and documentation are available.

Number of amino acids: 602

Molecular weight: 67293.35

Theoretical pI: 9.40

Amino acid composition: 
Ala (A)  22	  3.7%
Arg (R)  43	  7.1%
Asn (N)  48	  8.0%
Asp (D)  34	  5.6%
Cys (C)   5	  0.8%
Gln (Q)  25	  4.2%
Glu (E)  30	  5.0%
Gly (G)  31	  5.1%
His (H)   9	  1.5%
Ile (I)  24	  4.0%
Leu (L)  63	 10.5%
Lys (K)  36	  6.0%
Met (M)  10	  1.7%
Phe (F)  28	  4.7%
Pro (P)  25	  4.2%
Ser (S)  90	 15.0%
Thr (T)  31	  5.1%
Trp (W)   0	  0.0%
Tyr (Y)  15	  2.5%
Val (V)  33	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      2917
Hydrogen    H	      4682
Nitrogen    N	       858
Oxygen      O	       940
Sulfur      S	        15

Formula: C2917H4682N858O940S15
Total number of atoms: 9412

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22600
Abs 0.1% (=1 g/l)   0.336, assuming all pairs of Cys residues form cystines


Ext. coefficient    22350
Abs 0.1% (=1 g/l)   0.332, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.90
This classifies the protein as unstable.



Aliphatic index: 75.91

Grand average of hydropathicity (GRAVY): -0.618