ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLLRMNALT SKARSIERLK QTLNILSIRN HRQFSTIQQG SKYTLGFKKY LTLLNGEVGS 

        70         80         90        100        110        120 
FFHDVPLDLN EHEKTVNMIV EVPRWTTGKF EISKELRFNP IVQDTKNGKL RFVNNIFPYH 

       130        140        150        160        170        180 
GYIHNYGAIP QTWEDPTIEH KLGKCDVALK GDNDPLDCCE IGSDVLEMGS IKKVKVLGSL 

       190        200        210        220        230        240 
ALIDDGELDW KVIVIDVNDP LSSKIDDLEK IEEYFPGILD TTREWFRKYK VPAGKPLNSF 

       250        260        270        280        290        300 
AFHEQYQNSN KTIQTIKKCH NSWKNLISGS LQEKYDNLPN TERAGNGVTL EDSVKPPSQI 

       310 
PPEVQKWYYV 

References and documentation are available.

Number of amino acids: 310

Molecular weight: 35572.60

Theoretical pI: 7.09

Amino acid composition: 
Ala (A)   8	  2.6%
Arg (R)  11	  3.5%
Asn (N)  22	  7.1%
Asp (D)  19	  6.1%
Cys (C)   4	  1.3%
Gln (Q)  12	  3.9%
Glu (E)  21	  6.8%
Gly (G)  19	  6.1%
His (H)   8	  2.6%
Ile (I)  23	  7.4%
Leu (L)  30	  9.7%
Lys (K)  29	  9.4%
Met (M)   4	  1.3%
Phe (F)  12	  3.9%
Pro (P)  16	  5.2%
Ser (S)  19	  6.1%
Thr (T)  17	  5.5%
Trp (W)   6	  1.9%
Tyr (Y)  11	  3.5%
Val (V)  19	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1602
Hydrogen    H	      2508
Nitrogen    N	       428
Oxygen      O	       472
Sulfur      S	         8

Formula: C1602H2508N428O472S8
Total number of atoms: 5018

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49640
Abs 0.1% (=1 g/l)   1.395, assuming all pairs of Cys residues form cystines


Ext. coefficient    49390
Abs 0.1% (=1 g/l)   1.388, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.28
This classifies the protein as stable.



Aliphatic index: 87.03

Grand average of hydropathicity (GRAVY): -0.530