ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLLRRQPRL LNKRSTWLFR NYVLANRDVS EPVSRPAAPM STLSIPGPAL AAATPVSAVS 

        70         80         90        100        110        120 
LVPRCTHSSL QGGGQGAGGR GLRGSATGGS AAGGIATKRQ YSGGAAPAGA AKPGGAAPAG 

       130        140        150        160        170        180 
AAKPGGAAPA GAAKPGGAAA AAAGSAKPGG ASTGKPASGN APATPPPPPP PPPAKSAPPV 

       190        200        210        220        230        240 
KAKKPRLKTF EIYRWKPGDQ PQTQTYEVDL EQCGAMVLDA LIKIKNEMDP TLTFRRSCRE 

       250        260        270        280        290        300 
GICGSCAMNI NGTNTLACVS SIDQNESKCC RIYPLPHLYV VRDLVPDMSQ FYDQYRSIQP 

       310        320        330        340        350        360 
WLQRKDLKRE AGTAQYLQSV DDRLVLDGLY ECILCACCQT SCPSYWWNSN KYLGPAVLMQ 

       370        380        390        400        410        420 
AYRWVIDSRD EATEQRLDFL KDPWKLYRCH SIMNCTNTCP KHLNPARAII QLKQLLVGLK 

       430 
KKGKPQLKTD ALFAGKA 

References and documentation are available.

Number of amino acids: 437

Molecular weight: 46521.57

Theoretical pI: 9.63

Amino acid composition: 
Ala (A)  57	 13.0%
Arg (R)  27	  6.2%
Asn (N)  15	  3.4%
Asp (D)  18	  4.1%
Cys (C)  16	  3.7%
Gln (Q)  21	  4.8%
Glu (E)  11	  2.5%
Gly (G)  40	  9.2%
His (H)   4	  0.9%
Ile (I)  15	  3.4%
Leu (L)  39	  8.9%
Lys (K)  29	  6.6%
Met (M)   8	  1.8%
Phe (F)   6	  1.4%
Pro (P)  42	  9.6%
Ser (S)  29	  6.6%
Thr (T)  21	  4.8%
Trp (W)   7	  1.6%
Tyr (Y)  14	  3.2%
Val (V)  18	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2043
Hydrogen    H	      3276
Nitrogen    N	       598
Oxygen      O	       596
Sulfur      S	        24

Formula: C2043H3276N598O596S24
Total number of atoms: 6537

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60360
Abs 0.1% (=1 g/l)   1.297, assuming all pairs of Cys residues form cystines


Ext. coefficient    59360
Abs 0.1% (=1 g/l)   1.276, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.58
This classifies the protein as unstable.



Aliphatic index: 73.18

Grand average of hydropathicity (GRAVY): -0.354