ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNLTEYSHIL FLSLCTLNFC LYSINSDDNG GWERGHATFY GGADASGTMG GACGYGNLHS 

        70         80         90        100        110        120 
QGYGLQTAAL STALFNSGQK CGACFELQCE DDPEWCIPGS IIVSATNFCP PNFALANDNG 

       130        140        150        160        170        180 
GWCNPPLKHF DLAEPAFLQI AQYRAGIVPV AFRRVPCEKG GGIRFTINGN PYFDLVLITN 

       190        200        210        220        230        240 
VGGAGDIRAV SLKGSKTDQW QSMSRNWGQN WQSNTYLRGQ SLSFQVTDSD GRTVVSYDVV 

       250 
PHDWQFGQTF EGGQF 

References and documentation are available.

Number of amino acids: 255

Molecular weight: 27748.89

Theoretical pI: 4.96

Amino acid composition: 
Ala (A)  18	  7.1%
Arg (R)   9	  3.5%
Asn (N)  17	  6.7%
Asp (D)  14	  5.5%
Cys (C)  10	  3.9%
Gln (Q)  15	  5.9%
Glu (E)   8	  3.1%
Gly (G)  32	 12.5%
His (H)   5	  2.0%
Ile (I)  11	  4.3%
Leu (L)  20	  7.8%
Lys (K)   5	  2.0%
Met (M)   3	  1.2%
Phe (F)  16	  6.3%
Pro (P)  11	  4.3%
Ser (S)  19	  7.5%
Thr (T)  14	  5.5%
Trp (W)   7	  2.7%
Tyr (Y)   9	  3.5%
Val (V)  12	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 14

Atomic composition:

Carbon      C	      1232
Hydrogen    H	      1830
Nitrogen    N	       336
Oxygen      O	       374
Sulfur      S	        13

Formula: C1232H1830N336O374S13
Total number of atoms: 3785

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52535
Abs 0.1% (=1 g/l)   1.893, assuming all pairs of Cys residues form cystines


Ext. coefficient    51910
Abs 0.1% (=1 g/l)   1.871, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.01
This classifies the protein as stable.



Aliphatic index: 68.12

Grand average of hydropathicity (GRAVY): -0.214