ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNNDPKFQVQ VDPNEDTEWN DILRQHGIIP EKPPSPSVEL ETALEEAIVK QYDNRLENKD 

        70         80         90        100        110        120 
LDELDELEDE EDEDFLNHYK QKRMAEIKKL SEKKKFGHVL PISKNEYENE VTKASKESYV 

       130        140        150        160        170        180 
LVHLSLQSSL QSRLLSSILI DLASKFPELK ICDIPAQRCI ENYPESNCPT LIIYHDTNVV 

       190        200        210        220        230        240 
KQFITLTQLG GNATTLKDVE TALADLNVIG FHDKRLIINN DEDEDLEQAR KLRFAKKSIR 

       250        260 
DGNRNNEKDK DEDDDDDEDD DDFYD 

References and documentation are available.

Number of amino acids: 265

Molecular weight: 30709.98

Theoretical pI: 4.49

Amino acid composition: 
Ala (A)  11	  4.2%
Arg (R)  10	  3.8%
Asn (N)  19	  7.2%
Asp (D)  33	 12.5%
Cys (C)   3	  1.1%
Gln (Q)  11	  4.2%
Glu (E)  30	 11.3%
Gly (G)   6	  2.3%
His (H)   6	  2.3%
Ile (I)  18	  6.8%
Leu (L)  28	 10.6%
Lys (K)  24	  9.1%
Met (M)   2	  0.8%
Phe (F)   8	  3.0%
Pro (P)  11	  4.2%
Ser (S)  15	  5.7%
Thr (T)  10	  3.8%
Trp (W)   1	  0.4%
Tyr (Y)   7	  2.6%
Val (V)  12	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1339
Hydrogen    H	      2116
Nitrogen    N	       362
Oxygen      O	       454
Sulfur      S	         5

Formula: C1339H2116N362O454S5
Total number of atoms: 4276

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16055
Abs 0.1% (=1 g/l)   0.523, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.519, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.97
This classifies the protein as unstable.



Aliphatic index: 84.98

Grand average of hydropathicity (GRAVY): -0.909