ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNNGSGRYLL TPPDDLHPYV PSSKPQEQVY PDFKPWEHTA AEDQILANFV AKGFYHTPMV 

        70         80         90        100        110        120 
NFESISARSS VHESLVTQSN ILSQQFDKII KIREDHINKI PSNSTTTLHG PGFQLPNRIT 

       130        140        150        160        170        180 
LTDHRKETWL HELSSSHTSL VKIGKFIPHG LKRRQVIEQC YLKFIPLKRA IWLIKCCYFI 

       190        200        210        220        230        240 
EWKSNHKKKR SNAAGADDAI SMHLLKDWTD TFVYILEKLI FDMTNHYNDS QQLRTWKRQI 

       250        260        270        280        290        300 
SYFLKLLGNC YSLRLINKEI FHHWLVEFIN KMENFEFLPL SLHILMIFWN DICQIDTNAP 

       310        320        330        340        350        360 
VAATITSSQK EPFFLVTKIT DMLLHKYYIV SSSKSMINDE NYIINDIKKN NKIKLNILKI 

       370        380        390        400        410        420 
LSSLILKIFQ EQSLEVFIFP TSNWEIYKPL LFEIVSNADT NQNSDMKKKL ELISYRNESL 

       430        440        450        460        470        480 
KNNSSIRNVI MSASNANDFQ LTIVTCKQFP KLSCIQLNCI DTQFTKLLDD NPTEFDWPTY 

       490        500        510        520        530        540 
VDQNPLTMHK IIQLILWSIH PSRQFDHYES NQLVAKLLLL RINSTDEDLH EFQIEDAIWS 

       550        560        570        580        590        600 
LVFQLAKNFS AQKRVVSYMM PSLYRLLNIL ITYGIIKVPT YIRKLISSGL LYLQDSNDKF 

       610        620        630        640        650        660 
VHVQLLINLK ISPLMKSQYN MVLRNVMEYD VKFYEIFNFD QLVEITEQIK MRILSNDITN 

       670        680        690        700        710        720 
LQLSKTPLSI KIMVAEWYLS HLCSGILSSV NRTVLLKIFK IFCIDLEVFH HFFKWIEFIV 

       730        740        750        760        770        780 
YHQLLSDIES LEALMDILLC YQKLFSQFIN DHILFTKTFI FIYKKVLKEK DVPAYNVTSF 

       790        800        810        820        830        840 
MPFWKFFMKN FPFVLKVDND LRIELQSVYN DEKLKTEKLK NDKSEVLKVY SMINNSNQAV 

       850        860        870        880        890        900 
GQTWNFPEVF QVNIRFLLHN SEIIDTNTSK QFQKARNNVM LLIATNLKEY NKFMSIFLKR 

       910        920        930        940        950        960 
KDFTNKNLIQ LISLKLLTFE VTQNVLGLEY IIRLLPINLE NNDGSYGLFL KYHKEQFIKS 

       970        980        990       1000       1010       1020 
NFEKILLTCY ELEKKYHGNE CEINYYEILL KILITYGSSP KLLATSTKII MLLLNDSVEN 

      1030       1040       1050       1060       1070       1080 
SSNILEDILY YSTCPSETDL NDIPLGSGQP DNDTVVTNDD KSDDDDHTVD EIDHVEYYVM 

      1090       1100       1110       1120       1130       1140 
MDFANLWVFQ AFTCFCIKKI MENNEPAMAM EDLKNFIFQI IEITNSNDLC SQIFDQLKDM 

      1150       1160       1170       1180       1190       1200 
QTIEMITQIV EKDFCTSCLQ NNNQKIDDNY IVVVIEIITS LSMRFQRETS GMIVISMENY 

      1210       1220       1230       1240       1250       1260 
HLLIKIIRQL SELNEGNLSK REIQIDAVLK IFSFHQDSIF QRIIADLSAD KPTSPFIDSI 

      1270       1280       1290       1300       1310       1320 
CKLFDKISFN LRLKLFLYEI LSSLKSFAIY SSTIDAPAFH TSGKVELPKK LLNLPPFQVS 

      1330       1340       1350       1360       1370       1380 
SFVKETKLHS GDYGEEEDAD QEESFSLNLG IGIVEIAHEN EQKWLIYDKK DHKYVCTFSM 

      1390       1400       1410       1420 
EPYHFISNYN TKYTDDMATG SNDTTAFNDS CVNLSLFDAR FERKNPH 

References and documentation are available.

Number of amino acids: 1427

Molecular weight: 166860.62

Theoretical pI: 6.06

Amino acid composition: 
Ala (A)  42	  2.9%
Arg (R)  35	  2.5%
Asn (N) 104	  7.3%
Asp (D)  86	  6.0%
Cys (C)  22	  1.5%
Gln (Q)  63	  4.4%
Glu (E)  85	  6.0%
Gly (G)  28	  2.0%
His (H)  40	  2.8%
Ile (I) 143	 10.0%
Leu (L) 163	 11.4%
Lys (K) 115	  8.1%
Met (M)  37	  2.6%
Phe (F)  89	  6.2%
Pro (P)  45	  3.2%
Ser (S) 117	  8.2%
Thr (T)  73	  5.1%
Trp (W)  18	  1.3%
Tyr (Y)  55	  3.9%
Val (V)  67	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 171
Total number of positively charged residues (Arg + Lys): 150

Atomic composition:

Carbon      C	      7606
Hydrogen    H	     11828
Nitrogen    N	      1912
Oxygen      O	      2182
Sulfur      S	        59

Formula: C7606H11828N1912O2182S59
Total number of atoms: 23587

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   182325
Abs 0.1% (=1 g/l)   1.093, assuming all pairs of Cys residues form cystines


Ext. coefficient   180950
Abs 0.1% (=1 g/l)   1.084, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.43
This classifies the protein as stable.



Aliphatic index: 100.19

Grand average of hydropathicity (GRAVY): -0.167