ProtParam
User-provided sequence:
10 20 30 40 50 60
MNNNSRNNEN RSTINRNKRQ LQQAKEKNEN IHIPRYIRNQ PWYYKDTPKE QEGKKPGNDD
70 80 90 100 110 120
TSTAEGGEKS DYLVHHRQKA KGGALDIDNN SEPKIGMGIK DEFKLIRPQK MSVRDSHSLS
130 140 150 160 170 180
FCRNCGEAGH KEKDCMEKPR KMQKLVPDLN SQKNNGTVLV RATDDDWDSR KDRWYGYSGK
190 200 210 220 230 240
EYNELISKWE RDKRNKIKGK DKSQTDETLW DTDEEIELMK LELYKDSVGS LKKDDADNSQ
250 260 270 280 290 300
LYRTSTRLRE DKAAYLNDIN STESNYDPKS RLYKTETLGA VDEKSKMFRR HLTGEGLKLN
310 320 330 340 350 360
ELNQFARSHA KEMGIRDEIE DKEKVQHVLV ANPTKYEYLK KKREQEETKQ PKIVSIGDLE
370 380
ARKVDGTKQS EEQRNHLKDL YG
References and
documentation are available.
Number of amino acids: 382
Molecular weight: 44636.73
Theoretical pI: 8.89
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 77
Atomic composition:
Carbon C 1917
Hydrogen H 3081
Nitrogen N 581
Oxygen O 626
Sulfur S 11
Formula: C
1917H
3081N
581O
626S
11
Total number of atoms: 6216
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49975
Abs 0.1% (=1 g/l) 1.120, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 1.117, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.37
This classifies the protein as unstable.
Aliphatic index: 57.70
Grand average of hydropathicity (GRAVY): -1.421