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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNNNSRNNEN RSTINRNKRQ LQQAKEKNEN IHIPRYIRNQ PWYYKDTPKE QEGKKPGNDD

70 80 90 100 110 120
TSTAEGGEKS DYLVHHRQKA KGGALDIDNN SEPKIGMGIK DEFKLIRPQK MSVRDSHSLS

130 140 150 160 170 180
FCRNCGEAGH KEKDCMEKPR KMQKLVPDLN SQKNNGTVLV RATDDDWDSR KDRWYGYSGK

190 200 210 220 230 240
EYNELISKWE RDKRNKIKGK DKSQTDETLW DTDEEIELMK LELYKDSVGS LKKDDADNSQ

250 260 270 280 290 300
LYRTSTRLRE DKAAYLNDIN STESNYDPKS RLYKTETLGA VDEKSKMFRR HLTGEGLKLN

310 320 330 340 350 360
ELNQFARSHA KEMGIRDEIE DKEKVQHVLV ANPTKYEYLK KKREQEETKQ PKIVSIGDLE

370 380
ARKVDGTKQS EEQRNHLKDL YG


References and documentation are available.

Number of amino acids: 382 Molecular weight: 44636.73 Theoretical pI: 8.89
Amino acid composition: 
Ala (A) 14 3.7% Arg (R) 27 7.1% Asn (N) 28 7.3% Asp (D) 33 8.6% Cys (C) 3 0.8% Gln (Q) 17 4.5% Glu (E) 37 9.7% Gly (G) 22 5.8% His (H) 9 2.4% Ile (I) 16 4.2% Leu (L) 28 7.3% Lys (K) 50 13.1% Met (M) 8 2.1% Phe (F) 4 1.0% Pro (P) 11 2.9% Ser (S) 25 6.5% Thr (T) 18 4.7% Trp (W) 5 1.3% Tyr (Y) 15 3.9% Val (V) 12 3.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 70 Total number of positively charged residues (Arg + Lys): 77 Atomic composition: Carbon C 1917 Hydrogen H 3081 Nitrogen N 581 Oxygen O 626 Sulfur S 11 Formula: C1917H3081N581O626S11 Total number of atoms: 6216 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 49975 Abs 0.1% (=1 g/l) 1.120, assuming all pairs of Cys residues form cystines Ext. coefficient 49850 Abs 0.1% (=1 g/l) 1.117, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.37 This classifies the protein as unstable. Aliphatic index: 57.70 Grand average of hydropathicity (GRAVY): -1.421