ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNNWQHFFNN PVDLSEHLKK PYFRFDNRDK EITAISFDEK ANLIWSGDSY GCISSYDPTF 

        70         80         90        100        110        120 
QLYTRYRGHI GGNSVKDILS HRDGILSISE DSLHFANRRG VTKLNLTSID IAAFSELNTM 

       130        140        150        160        170        180 
CYSPHSLKNN IYCGGDNTNW GIASIDLNRG CLDSLLNYSS KVKLMCSNNK VLSIGRQTGT 

       190        200        210        220        230        240 
VDLLDPTSNR TIKSFNAHSA SISAMDLRDN TLVTVGKSKR FYNLYADPFV NVYDLRTMRQ 

       250        260        270        280        290        300 
LPPVSFSKGT TMGSGGADFV QLHPLLPTVM IVASSSGSFD FIDLSNPTLR TQYVHPCQSI 

       310        320        330        340        350        360 
KKLCLSPNGD VLGILEADNH LDTWRRSSNN MGMFTNTPEM LAYPDYFNDI TSDGPISVDD 

       370        380        390        400        410        420 
ETYPLSSVGM PYYLDKLLSA WPPVVFKSEG TIPQLTGKSP LPSSGKLKSN LAVISSQNEK 

       430        440        450        460        470        480 
LSTQEFPLLR YDRTKYGMRN AIPDYVCLRD IRKQITSGLE TSDIQTYTSI NKYEVPPAYS 

       490        500        510        520        530        540 
RLPLTSGRFG TDNFDFTPFN NTEYSGLDPD VDNHYTNAII QLYRFIPEMF NFVVGCLKDE 

       550        560        570        580        590        600 
NFETTLLTDL GYLFDMMERS HGKICSSSNF QASLKSLTDK RQLENGEPQE HLEEYLESLC 

       610        620        630        640        650        660 
IRESIEDFNS SESIKRNMPQ KFNRFLLSQL IKEEAQTVNH NITLNQCFGL ETEIRTECSC 

       670        680        690        700        710        720 
DHYDTTVKLL PSLSISGINK TVIKQLNKKS NGQNILPYIE YAMKNVTQKN SICPTCGKTE 

       730        740        750        760        770        780 
TITQECTVKN LPSVLSLELS LLDTEFSNIR SSKNWLTSEF YGSIIKNKAV LRSTASELKG 

       790        800        810        820        830        840 
TSHIFKYELN GYVAKITDNN NETRLVTYVK KYNPKENCFK WLMFNDYLVV EITEEEALKM 

       850        860        870        880        890        900 
TYPWKTPEII IYCDAEELRK PFFSVDTYSI NYDILFRDYF ANGIRDTARR EYKLLTHDEA 

       910        920        930        940        950        960 
PKSGTLVAID AEFVSLQSEL CEIDHQGIRS IIRPKRTALA RISIIRGEEG ELYGVPFVDD 

       970        980        990       1000       1010       1020 
YVVNTNHIED YLTRYSGILP GDLDPEKSTK RLVRRNVVYR KVWLLMQLGC VFVGHGLNND 

      1030       1040       1050       1060       1070       1080 
FKHININVPR NQIRDTAIYF LQGKRYLSLR YLAYVLLGMN IQEGNHDSIE DAHTALILYK 

      1090       1100       1110 
KYLHLKEKAI FEKVLNSVYE EGRAHNFKVP ETSKG 

References and documentation are available.

Number of amino acids: 1115

Molecular weight: 127039.07

Theoretical pI: 6.25

Amino acid composition: 
Ala (A)  40	  3.6%
Arg (R)  54	  4.8%
Asn (N)  80	  7.2%
Asp (D)  67	  6.0%
Cys (C)  21	  1.9%
Gln (Q)  30	  2.7%
Glu (E)  66	  5.9%
Gly (G)  59	  5.3%
His (H)  25	  2.2%
Ile (I)  76	  6.8%
Leu (L) 115	 10.3%
Lys (K)  69	  6.2%
Met (M)  21	  1.9%
Phe (F)  49	  4.4%
Pro (P)  48	  4.3%
Ser (S) 100	  9.0%
Thr (T)  75	  6.7%
Trp (W)   9	  0.8%
Tyr (Y)  55	  4.9%
Val (V)  56	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 123

Atomic composition:

Carbon      C	      5663
Hydrogen    H	      8833
Nitrogen    N	      1515
Oxygen      O	      1722
Sulfur      S	        42

Formula: C5663H8833N1515O1722S42
Total number of atoms: 17775

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   132700
Abs 0.1% (=1 g/l)   1.045, assuming all pairs of Cys residues form cystines


Ext. coefficient   131450
Abs 0.1% (=1 g/l)   1.035, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.65
This classifies the protein as stable.



Aliphatic index: 84.96

Grand average of hydropathicity (GRAVY): -0.394