ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNPADLNSTS VPLLRNQNIT QKNNRQWKNF IKHCYILITI LVLSAPIAFS LSKLIMVARH 

        70         80         90        100        110        120 
KSPTVSNLIR VFIPSSKKLK TTTEKNLPTT KQLFKYSTET IFPPAALCGK RIVGYFAEFE 

       130        140        150        160        170        180 
NTALTRKQLQ MLTHIIYLFA IPKNGNMTFG GERSRRKFEE MKNEARKANS TLKVMISIGG 

       190        200        210        220        230        240 
QSNSRSFSRL VSNETSRNVF VNSIVSFVQK YDIDGIEIFW TWPKYEDANN YLIFIQELRY 

       250        260        270        280        290        300 
AFIELQKKLN RKEDYVISII VNCYDNQLSN LMGFSKFVDF FNIYSIHYQS RQVGPSSPLY 

       310        320        330        340        350        360 
GGGSRNIDET MKYYICRTGQ PSKFNMMVLF HGTFWKGAEL PLRNDSDDIF KDQNSAKGGF 

       370        380        390        400        410        420 
AVRWRELLQQ KWDMSNIKFH NLTKTSYMWI PGSSLFLTLE DEQSLRVKNR YVADHNIGGI 

       430        440        450 
TMWTIDQDDG DHTLLKVVSS AELCTGDEKN TIKFKCE 

References and documentation are available.

Number of amino acids: 457

Molecular weight: 52772.69

Theoretical pI: 9.48

Amino acid composition: 
Ala (A)  18	  3.9%
Arg (R)  23	  5.0%
Asn (N)  34	  7.4%
Asp (D)  20	  4.4%
Cys (C)   6	  1.3%
Gln (Q)  19	  4.2%
Glu (E)  22	  4.8%
Gly (G)  23	  5.0%
His (H)   8	  1.8%
Ile (I)  37	  8.1%
Leu (L)  39	  8.5%
Lys (K)  37	  8.1%
Met (M)  13	  2.8%
Phe (F)  28	  6.1%
Pro (P)  15	  3.3%
Ser (S)  39	  8.5%
Thr (T)  29	  6.3%
Trp (W)   8	  1.8%
Tyr (Y)  17	  3.7%
Val (V)  22	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2379
Hydrogen    H	      3716
Nitrogen    N	       640
Oxygen      O	       680
Sulfur      S	        19

Formula: C2379H3716N640O680S19
Total number of atoms: 7434

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69705
Abs 0.1% (=1 g/l)   1.321, assuming all pairs of Cys residues form cystines


Ext. coefficient    69330
Abs 0.1% (=1 g/l)   1.314, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.41
This classifies the protein as unstable.



Aliphatic index: 82.76

Grand average of hydropathicity (GRAVY): -0.355