ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNPADSNSTS VPLLRNQNLI PNRRQPTQWK HLAKPCLILI AILVLSAPVA YSLSELIMVA 

        70         80         90        100        110        120 
RHKSPAVSNL IELLIPSSKT SRTTTKTNDS TMTKLVQKSD ENFSPPAAFC GKRIVGYFAE 

       130        140        150        160        170        180 
FENTALTRKQ LRMLTHIVFL FAFPKNGTIT FGGESSSQKF EEMRRNVRKA SSTLKVMISI 

       190        200        210        220        230        240 
GGQYNSGEFS GLVSNETSRN LFVNSIATFV RDYDIDGVDI FWTWPKHSDE NNYLMFIREL 

       250        260        270        280        290        300 
RYAFTELQKK LNRKETFVIS LVISRNVNHL SKLVEFSNFV DFINIYSFNS YLYQVGPDSP 

       310        320        330        340        350        360 
LYGGGSRNVD EIMKYYICKT GQPSKFNIIV SFHATYWEGA ELPLRDDSDD IFKDQNSAKG 

       370        380        390        400        410        420 
GFAVRWRELL QQKWDMSNIK FHNLTKTSYM WIPGPPTRFM TLEDEKSLRE KNRYVADHNI 

       430        440        450        460 
GGITMWTIDQ DDGDHTLLKV VSSAELCTGD RRNEIKYKCD 

References and documentation are available.

Number of amino acids: 460

Molecular weight: 52650.06

Theoretical pI: 9.02

Amino acid composition: 
Ala (A)  21	  4.6%
Arg (R)  26	  5.7%
Asn (N)  31	  6.7%
Asp (D)  25	  5.4%
Cys (C)   5	  1.1%
Gln (Q)  14	  3.0%
Glu (E)  24	  5.2%
Gly (G)  23	  5.0%
His (H)   9	  2.0%
Ile (I)  31	  6.7%
Leu (L)  40	  8.7%
Lys (K)  31	  6.7%
Met (M)  12	  2.6%
Phe (F)  26	  5.7%
Pro (P)  19	  4.1%
Ser (S)  43	  9.3%
Thr (T)  30	  6.5%
Trp (W)   8	  1.7%
Tyr (Y)  16	  3.5%
Val (V)  26	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2360
Hydrogen    H	      3678
Nitrogen    N	       640
Oxygen      O	       693
Sulfur      S	        17

Formula: C2360H3678N640O693S17
Total number of atoms: 7388

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68090
Abs 0.1% (=1 g/l)   1.293, assuming all pairs of Cys residues form cystines


Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.289, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.62
This classifies the protein as unstable.



Aliphatic index: 81.15

Grand average of hydropathicity (GRAVY): -0.374