ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNPIKAQSTG SPKKFNVFAH VKYEHLVAGV SGGVVSTLIL HPLDLIKIRF AVNDGRTATV 

        70         80         90        100        110        120 
PQYRGLSSAF TTIFRQEGFR GLYKGVTPNV WGSGSSWGLY FMFYNTIKTF IQGGNTTMPL 

       130        140        150        160        170        180 
GPTMNMLAAA ESGILTLLLT NPIWVVKTRL CLQCDAASSA EYRGMIHALG QIYKEEGIRG 

       190        200        210        220        230        240 
LYRGFVPGML GVSHGAIQFM TYEELKNAYN EYRKLPIDTK LATTEYLAFA AVSKLIAAAA 

       250        260        270        280        290        300 
TYPYQVVRAR LQDHHHRYNG TWDCIKQTWR YERMRGFYKG LVPYLVHVTP NICMVMLIWE 


KLTS 

References and documentation are available.

Number of amino acids: 304

Molecular weight: 34157.74

Theoretical pI: 9.60

Amino acid composition: 
Ala (A)  24	  7.9%
Arg (R)  16	  5.3%
Asn (N)  12	  3.9%
Asp (D)   6	  2.0%
Cys (C)   4	  1.3%
Gln (Q)  10	  3.3%
Glu (E)  12	  3.9%
Gly (G)  27	  8.9%
His (H)   9	  3.0%
Ile (I)  18	  5.9%
Leu (L)  29	  9.5%
Lys (K)  16	  5.3%
Met (M)  11	  3.6%
Phe (F)  13	  4.3%
Pro (P)  13	  4.3%
Ser (S)  15	  4.9%
Thr (T)  24	  7.9%
Trp (W)   6	  2.0%
Tyr (Y)  18	  5.9%
Val (V)  21	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1559
Hydrogen    H	      2414
Nitrogen    N	       414
Oxygen      O	       420
Sulfur      S	        15

Formula: C1559H2414N414O420S15
Total number of atoms: 4822

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60070
Abs 0.1% (=1 g/l)   1.759, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   1.751, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 19.44
This classifies the protein as stable.



Aliphatic index: 88.22

Grand average of hydropathicity (GRAVY): -0.008