ProtParam
User-provided sequence:
10 20 30 40 50 60
MNPSAPSYPM ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RVCRDMITRR SLGYAYVNFQ
70 80 90 100 110 120
QPADAERALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN IFIKNLDKSI DNKALYDTFS
130 140 150 160 170 180
AFGNILSCKV VCDENGSKGY GFVHFETQEA AERAIEKMNG MLLNDRKVFV GRFKSRKERE
190 200 210 220 230 240
AELGARAKEF TNVYIKNFGE DMDDERLKDL FGKFGPALSV KVMTDESGKS KGFGFVSFER
250 260 270 280 290 300
HEDAQKAVDE MNGKELNGKQ IYVGRAQKKV ERQTELKRKF EQMKQDRITR YQGVNLYVKN
310 320 330 340 350 360
LDDGIDDERL RKEFSPFGTI TSAKVMMEGG RSKGFGFVCF SSPEEATKAV TEMNGRIVAT
370 380 390 400 410 420
KPLYVALAQR KEERQAHLTN QYMQRMASVR AVPNPVINPY QPAPPSGYFM AAIPQTQNRA
430 440 450 460 470 480
AYYPPSQIAQ LRPSPRWTAQ GARPHPFQNM PGAIRPAAPR PPFSTMRPAS SQVPRVMSTQ
490 500 510 520 530 540
RVANTSTQTM GPRPAAAAAA ATPAVRTVPQ YKYAAGVRNP QQHLNAQPQV TMQQPAVHVQ
550 560 570 580 590 600
GQEPLTASML ASAPPQEQKQ MLGERLFPLI QAMHPTLAGK ITGMLLEIDN SELLHMLESP
610 620 630
ESLRSKVDEA VAVLQAHQAK EAAQKAVNSA TGVPTV
References and
documentation are available.
Number of amino acids: 636
Molecular weight: 70670.84
Theoretical pI: 9.52
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 84
Atomic composition:
Carbon C 3109
Hydrogen H 4946
Nitrogen N 896
Oxygen O 923
Sulfur S 32
Formula: C
3109H
4946N
896O
923S
32
Total number of atoms: 9906
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39560
Abs 0.1% (=1 g/l) 0.560, assuming all pairs of Cys residues form cystines
Ext. coefficient 39310
Abs 0.1% (=1 g/l) 0.556, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.87
This classifies the protein as unstable.
Aliphatic index: 68.62
Grand average of hydropathicity (GRAVY): -0.511