ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNPSLNFIHV SRVTPSNSNS SASLTLPLTF FDLLWLKHKA VERVIFYKLT DVNRSLFDSV 

        70         80         90        100        110        120 
IVPNLKSSLS SSLSHYLPLA GHIIWEPHDP KPKIVYTQND AVSFTVAESN SDFSLLTGKE 

       130        140        150        160        170        180 
PFSSTELHPL VPELQNSDDS AAVVSFQVTL FPNQGFCIGV TTHHAVSDGK TTTTFLKSWA 

       190        200        210        220        230        240 
HLCKHQDSSL PDDLIPFYDR TVIKGPPEID TKVLKIWHSI HKPKSLKLLP RPEIESDVVR 

       250        260        270        280        290        300 
YTFELTRENI EKLRDKLKRE SSSFSSVRLS TFVITFSYVF TCLIGSGGDD PNRPVGYRFA 

       310        320        330        340        350        360 
VDCRRLIDDP PIPLTYFGNC VYSAVKIPLD AGMFLGEQGF VVAARLISDS VEELDSNVAW 

       370        380        390        400        410        420 
KIPELLETYE KAPVDSQFVS VAGSTRFGIY GLDFGWGKPF KSLLVSIDQR GKISIAESRD 

       430        440 
GSGGVEIGFS LKKQEMNVLI DLLHKGIK 

References and documentation are available.

Number of amino acids: 448

Molecular weight: 49916.07

Theoretical pI: 6.36

Amino acid composition: 
Ala (A)  18	  4.0%
Arg (R)  18	  4.0%
Asn (N)  15	  3.3%
Asp (D)  29	  6.5%
Cys (C)   5	  1.1%
Gln (Q)   9	  2.0%
Glu (E)  22	  4.9%
Gly (G)  25	  5.6%
His (H)  13	  2.9%
Ile (I)  28	  6.2%
Leu (L)  48	 10.7%
Lys (K)  29	  6.5%
Met (M)   3	  0.7%
Phe (F)  27	  6.0%
Pro (P)  28	  6.2%
Ser (S)  52	 11.6%
Thr (T)  25	  5.6%
Trp (W)   6	  1.3%
Tyr (Y)  11	  2.5%
Val (V)  37	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      2270
Hydrogen    H	      3537
Nitrogen    N	       587
Oxygen      O	       663
Sulfur      S	         8

Formula: C2270H3537N587O663S8
Total number of atoms: 7065

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49640
Abs 0.1% (=1 g/l)   0.994, assuming all pairs of Cys residues form cystines


Ext. coefficient    49390
Abs 0.1% (=1 g/l)   0.989, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.84
This classifies the protein as stable.



Aliphatic index: 94.13

Grand average of hydropathicity (GRAVY): -0.093