ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNPTFLEFIG ANQRSSCGYC KNDRGERTPA RSAKKNAETG EEDLTNGLEE ELEVSSESSV 

        70         80         90        100        110        120 
TTGGAWAHSM TVNDYSGLLD VGWRRSGRYL YKPDNRVTCC PQYTIRLDVT KFKMSRSQKR 

       130        140        150        160        170        180 
AMRQMNEFLA TGKRPVCGIK EEDAPVEMNL KGSGSQSEQS KKNLKSHEVS TKKMKDADRP 

       190        200        210        220        230        240 
VLTKKEIRNK KFEEKCRQKN LDPDVVRTER RQKDAARQRT IQSYIDEARP DWKHKLEVKL 

       250        260        270        280        290        300 
VSLGGDEFGT RDNESFELYK NYQHTIHKDE DCRLAGFRRF LCDSPLKKEQ RGGIELGSFH 

       310        320        330        340        350        360 
LWFLLDDKLI AVCVVDILPK CFSAKYMYYN PEYSFLSLGT YTALREIEQT QRLHAIYSNL 

       370        380        390        400        410        420 
KYYYMGYYIH SCPKMRYKAK FRPSDLLCDQ SFRWVDFNSC RDLLDGSTCS NGFSEFCPGA 

       430        440        450        460        470        480 
PRPASPVVDT LLVFSNGSIT TLSDALTEIP EAAENEQFLA NVRDLAHLVG PDLSRVVYYF 


NELNS 

References and documentation are available.

Number of amino acids: 485

Molecular weight: 55660.94

Theoretical pI: 8.36

Amino acid composition: 
Ala (A)  26	  5.4%
Arg (R)  36	  7.4%
Asn (N)  23	  4.7%
Asp (D)  31	  6.4%
Cys (C)  15	  3.1%
Gln (Q)  16	  3.3%
Glu (E)  37	  7.6%
Gly (G)  28	  5.8%
His (H)   9	  1.9%
Ile (I)  15	  3.1%
Leu (L)  44	  9.1%
Lys (K)  37	  7.6%
Met (M)  10	  2.1%
Phe (F)  21	  4.3%
Pro (P)  20	  4.1%
Ser (S)  40	  8.2%
Thr (T)  25	  5.2%
Trp (W)   5	  1.0%
Tyr (Y)  22	  4.5%
Val (V)  25	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      2443
Hydrogen    H	      3826
Nitrogen    N	       692
Oxygen      O	       748
Sulfur      S	        25

Formula: C2443H3826N692O748S25
Total number of atoms: 7734

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61155
Abs 0.1% (=1 g/l)   1.099, assuming all pairs of Cys residues form cystines


Ext. coefficient    60280
Abs 0.1% (=1 g/l)   1.083, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.03
This classifies the protein as unstable.



Aliphatic index: 67.75

Grand average of hydropathicity (GRAVY): -0.688