ProtParam
User-provided sequence:
10 20 30 40 50 60
MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD
70 80 90 100 110 120
EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV
130 140 150 160 170 180
NDISIFFPNG ECNRARYLPQ NPDIIAGASS DGAIYIFDRT KHGSTRIRQS KISHPFETKL
190 200 210 220 230 240
FGSHGVIQDV EAMDTSSADI NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII
250 260 270 280 290 300
DLPLVSINSD GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN
310 320 330 340 350 360
SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP NFDTVLATAG
370 380 390 400 410 420
QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM CSVANDNSVH IWKPAGNLVG
HS
References and
documentation are available.
Number of amino acids: 422
Molecular weight: 47364.71
Theoretical pI: 5.32
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 2080
Hydrogen H 3200
Nitrogen N 576
Oxygen O 661
Sulfur S 16
Formula: C
2080H
3200N
576O
661S
16
Total number of atoms: 6533
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78295
Abs 0.1% (=1 g/l) 1.653, assuming all pairs of Cys residues form cystines
Ext. coefficient 77920
Abs 0.1% (=1 g/l) 1.645, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.36
This classifies the protein as stable.
Aliphatic index: 76.28
Grand average of hydropathicity (GRAVY): -0.502