ProtParam
User-provided sequence:
10 20 30 40 50 60
MNQEDNTGGG GIFGLFKWTK DALFGTDISP SMKYKDQEER RDRSRYAQDD TNFSMKFGND
70 80 90 100 110 120
SNRRSTNLSR SNSWSGLDST LHRKYELLPE YNENGFNSIV NGDHHSKERI RSLRSPAPIV
130 140 150 160 170 180
PREPLRNEPT DTFGHRLHTK RRTINELSNS QIPFIPPQED DPLLSKLFNK DGVNEVRRSP
190 200 210 220 230 240
YKLSVKDIPG KFPSPLTKRD EIDNYYVRDE DACHKNREYK KAYFDLFAQM DLNSRDLEDL
250 260 270 280 290 300
CEDVREQREQ FHRNEQTYKQ AYEEMRAELV NELKKSKTLF ENYYSLGQKY KSLKKVLDQT
310 320 330 340 350 360
ISHEAELATS RERLYQEEDL KNFEIQTLKQ RLSDLELKYT NLQIEKDMQR DNYESEIHDL
370 380
LLQLSLRNNE RKDTSAGSNI FSTGQ
References and
documentation are available.
Number of amino acids: 385
Molecular weight: 45384.31
Theoretical pI: 6.08
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 1976
Hydrogen H 3098
Nitrogen N 578
Oxygen O 636
Sulfur S 8
Formula: C
1976H
3098N
578O
636S
8
Total number of atoms: 6296
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36455
Abs 0.1% (=1 g/l) 0.803, assuming all pairs of Cys residues form cystines
Ext. coefficient 36330
Abs 0.1% (=1 g/l) 0.800, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.99
This classifies the protein as unstable.
Aliphatic index: 64.34
Grand average of hydropathicity (GRAVY): -1.164