ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNQKLLKLEK LLRFHTICRQ LHSLSQRRLL AWWKHGSAPA SPVWADWLCA RPWPTDVLTG 

        70         80         90        100        110        120 
SALHQHRLLV TKKEKRPPRS QLSPIKTKKE VEVWVGMTVE ELARAMAKDT DCVYEALLNT 

       130        140        150        160        170        180 
AIDIDSLEAN SHLDEVWIKE VIKKAGMKLK WSKLKLERIR ENKDAVRRPG ADPALLKPRS 

       190        200        210        220        230        240 
PVVTIMGHVD HGKTTLLDKL RETQVAAMEV GGITQHIGAF LVSLPSGEKI TFLDTPGHAA 

       250        260        270        280        290        300 
FSAMRARGAQ VTDIVVLVVA ADDGVMKQTV ESIQHAKDAG VPIILAINKC DKTDADPEKV 

       310        320        330        340        350        360 
KKELLAYDVV CEEYGGDVQA VHVSALTGKN LMALAEATIA LAEMLELKAD PTGPVEGTVI 

       370        380        390        400        410        420 
ESFTDKGRGP VTTAIIQRGT LKKGSILVAG KSWAKVRLMF DENGKTVNEA YPSMPVGIIG 

       430        440        450        460        470        480 
WRDLPSAGDE ILEVQSEPRA REVVDWRKSE QKEEKGKDDL KIMEEKRKEH QEAHRKAREK 

       490        500        510        520        530        540 
YGSLHWKQRS YIKYLERKEQ RPLKPKEKVE RDSNVLPIII KGDVDGSVEA ILNLLDTYDA 

       550        560        570        580        590        600 
SHECELELVH FGLGDISEND VTFAETFDGV IYGFNVDAGS AIQQSAAQKG VKIKLHKIIY 

       610        620        630        640        650        660 
HLIEDLQEEL SNRLPYKVEE YPIGEASILA TFTITEGKKK VPVAGCRVQK GQLERQKKFK 

       670        680        690        700        710        720 
LIRNGQVIWK GSLTSLKHHK DDVLVIKTGV DCGLSLDEEN VEFKVGDQVI CYEENKVPTK 


TSWDPGF 

References and documentation are available.

Number of amino acids: 727

Molecular weight: 81254.67

Theoretical pI: 7.62

Amino acid composition: 
Ala (A)  57	  7.8%
Arg (R)  35	  4.8%
Asn (N)  16	  2.2%
Asp (D)  45	  6.2%
Cys (C)   9	  1.2%
Gln (Q)  26	  3.6%
Glu (E)  62	  8.5%
Gly (G)  49	  6.7%
His (H)  21	  2.9%
Ile (I)  44	  6.1%
Leu (L)  70	  9.6%
Lys (K)  73	 10.0%
Met (M)  13	  1.8%
Phe (F)  14	  1.9%
Pro (P)  30	  4.1%
Ser (S)  37	  5.1%
Thr (T)  37	  5.1%
Trp (W)  14	  1.9%
Tyr (Y)  13	  1.8%
Val (V)  62	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      3619
Hydrogen    H	      5851
Nitrogen    N	      1003
Oxygen      O	      1071
Sulfur      S	        22

Formula: C3619H5851N1003O1071S22
Total number of atoms: 11566

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    96870
Abs 0.1% (=1 g/l)   1.192, assuming all pairs of Cys residues form cystines


Ext. coefficient    96370
Abs 0.1% (=1 g/l)   1.186, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.16
This classifies the protein as stable.



Aliphatic index: 93.73

Grand average of hydropathicity (GRAVY): -0.372