ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNQTTETDSM RINLQRTYKD AINALNSLQS NFASIEATKK LGPSVNRNEL SINEVHEFTK 

        70         80         90        100        110        120 
RLGYTPTDFN KLNIIHITGT KGKGSTCAFT ESILKQYPIS KIGLYTSPHL KSVRERIRIN 

       130        140        150        160        170        180 
GQPINQEKFA KYFFEVWDKF TTTKSDPQEW PTLQPCDQVK PMYFKYLTIL SFHVFLQEGV 

       190        200        210        220        230        240 
DTAIYEVGVG GTYDSTNIID KPTVTGISAL GIDHTFMLGD NIASITENKT GIFKKGVPAF 

       250        260        270        280        290        300 
VSKQSEYPET HELIEKRAKQ LGVSSLEFVD TEDLPNVKLG LSGEFQKQNA ALAIRIANSH 

       310        320        330        340        350        360 
LKTIGITQDL PEFNNNDGKI KKLSNEFIKG LENVDWPGRC QIINNNPTGI TWYIDGAHTI 

       370        380        390        400        410        420 
ESINASSTWF KQEQLKLEKP KRRALLFNQQ GRENYAELLE KLFNVTYGTG SEPQIKFDDV 

       430        440        450        460        470        480 
IFTTNTTWSS GQFNSELISK NTSEDAVKKL EVQNNLSEVW GKLDGGVSKR HVFADIETAV 

       490        500        510 
NYLKDLGDED LQVFVCGSLH LVGGFLVVLD NERD 

References and documentation are available.

Number of amino acids: 514

Molecular weight: 57836.33

Theoretical pI: 5.90

Amino acid composition: 
Ala (A)  22	  4.3%
Arg (R)  15	  2.9%
Asn (N)  37	  7.2%
Asp (D)  27	  5.3%
Cys (C)   4	  0.8%
Gln (Q)  24	  4.7%
Glu (E)  37	  7.2%
Gly (G)  36	  7.0%
His (H)  10	  1.9%
Ile (I)  39	  7.6%
Leu (L)  45	  8.8%
Lys (K)  42	  8.2%
Met (M)   4	  0.8%
Phe (F)  27	  5.3%
Pro (P)  18	  3.5%
Ser (S)  35	  6.8%
Thr (T)  42	  8.2%
Trp (W)   7	  1.4%
Tyr (Y)  14	  2.7%
Val (V)  29	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2591
Hydrogen    H	      4057
Nitrogen    N	       689
Oxygen      O	       795
Sulfur      S	         8

Formula: C2591H4057N689O795S8
Total number of atoms: 8140

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59610
Abs 0.1% (=1 g/l)   1.031, assuming all pairs of Cys residues form cystines


Ext. coefficient    59360
Abs 0.1% (=1 g/l)   1.026, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.68
This classifies the protein as stable.



Aliphatic index: 84.38

Grand average of hydropathicity (GRAVY): -0.437