ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRFWNTKKF SLTNADGLCA TLNEISQNDE VLVVQPSVLP VLNSLLTFQD LTQSTPVRKI 

        70         80         90        100        110        120 
TLLDDQLSDD LPSALGSVPQ MDLIFLIDVR TSLRLPPQLL DAAQKHNLSS LHIIYCRWKP 

       130        140        150        160        170        180 
SFQNTLEDTE QWQKDGFDLN SKKTHFPNVI ESQLKELSNE YTLYPWDLLP FPQIDENVLL 

       190        200        210        220        230        240 
THSLYNMENV NMYYPNLRSL QSATESILVD DMVNSLQSLI FETNSIITNV VSIGNLSKRC 

       250        260        270        280        290        300 
SHLLKKRIDE HQTENDLFIK GTLYGERTNC GLEMDLIILE RNTDPITPLL TQLTYAGILD 

       310        320        330        340        350        360 
DLYEFNSGIK IKEKDMNFNY KEDKIWNDLK FLNFGSIGPQ LNKLAKELQT QYDTRHKAES 

       370        380        390        400        410        420 
VHEIKEFVDS LGSLQQRQAF LKNHTTLSSD VLKVVETEEY GSFNKILELE LEILMGNTLN 

       430        440        450        460        470        480 
NDIEDIILEL QYQYEVDQKK ILRLICLLSL CKNSLREKDY EYLRTFMIDS WGIEKCFQLE 

       490        500        510        520        530        540 
SLAELGFFTS KTGKTDLHIT TSKSTRLQKE YRYISQWFNT VPIEDEHAAD KITNENDDFS 

       550        560        570        580        590        600 
EATFAYSGVV PLTMRLVQML YDRSILFHNY SSQQPFILSR EPRVSQTEDL IEQLYGDSHA 

       610        620        630        640        650        660 
IEESIWVPGT ITKKINASIK SNNRRSIDGS NGTFHAAEDI ALVVFLGGVT MGEIAIMKHL 

       670        680        690 
QKILGKKGIN KRFIIIADGL INGTRIMNSI S 

References and documentation are available.

Number of amino acids: 691

Molecular weight: 79271.31

Theoretical pI: 5.13

Amino acid composition: 
Ala (A)  22	  3.2%
Arg (R)  25	  3.6%
Asn (N)  46	  6.7%
Asp (D)  45	  6.5%
Cys (C)   7	  1.0%
Gln (Q)  35	  5.1%
Glu (E)  49	  7.1%
Gly (G)  29	  4.2%
His (H)  15	  2.2%
Ile (I)  57	  8.2%
Leu (L)  91	 13.2%
Lys (K)  44	  6.4%
Met (M)  14	  2.0%
Phe (F)  29	  4.2%
Pro (P)  21	  3.0%
Ser (S)  56	  8.1%
Thr (T)  46	  6.7%
Trp (W)   8	  1.2%
Tyr (Y)  22	  3.2%
Val (V)  30	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3545
Hydrogen    H	      5603
Nitrogen    N	       929
Oxygen      O	      1085
Sulfur      S	        21

Formula: C3545H5603N929O1085S21
Total number of atoms: 11183

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    77155
Abs 0.1% (=1 g/l)   0.973, assuming all pairs of Cys residues form cystines


Ext. coefficient    76780
Abs 0.1% (=1 g/l)   0.969, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.23
This classifies the protein as unstable.



Aliphatic index: 99.31

Grand average of hydropathicity (GRAVY): -0.303