ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRGSLDDGP KLREEKHFQD FYPDLNADTL LPFIVPLVET KDNSTDTDSD DISNRNNREI 

        70         80         90        100        110        120 
GSVKSVQTKE LIFKGRVTTE PLVLKKNEVE FQKCKITTNE LKGKKNPYCV RFNESFISRY 

       130        140        150        160        170        180 
YHINKVRNRK SYKQQQKEFD GVEAPYFTKF SSKEAPNITI STSTKSAIQK FASISPNLVN 

       190        200        210        220        230        240 
FKPQYDMDEQ DELYLHYLNK RYFKDQMSHE IFEILMTTLE TEWFHIEKHI PSTNSLIARH 

       250        260        270        280        290        300 
NILRDCKNYE LYGSDDGTGL SMDQACAVCL GTDSDNLNTI VFCDGCDIAV HQECYGIIFI 

       310        320        330        340        350        360 
PEGKWLCRRC MISKNNFATC LMCPSHTGAF KQTDTGSWVH NICALWLPEL YFSNLHYMEP 

       370        380        390        400        410        420 
IEGVQNVSVS RWKLNCYICK KKMGACIQCF QRNCFTAYHV TCARRAGLYM SKGKCTIQEL 

       430        440        450        460        470        480 
ASNQFSQKYS VESFCHKHAP RGWQTSIEGI NKARKYFSLL STLQTETPQH NEANDRTNSK 

       490        500        510        520        530        540 
FNKTIWKTPN QTPVAPHVFA EILQKVVDFF GLANPPAGAF DICKYWSMKR ELTGGTPLTA 

       550        560        570        580        590        600 
CFENNSLGSL TEEQVQTRID FANDQLEDLY RLKELTTLVK KRTQASNSLS RSRKKVFDIV 

       610        620        630        640        650        660 
KSPQKYLLKI NVLDIFIKSE QFKALERLVT EPKLLVILEK CKHCDFDTVQ IFKEEIMHFF 

       670        680        690        700        710        720 
EVLETLPGAS RILQTVSSKA KEQVTNLIGL IEHVDIKKLL SRDFIINDDK IEERPWSGPV 

       730        740 
IMEEEGLSDA EELSAGEHRM LKLILNSG 

References and documentation are available.

Number of amino acids: 748

Molecular weight: 86029.35

Theoretical pI: 7.69

Amino acid composition: 
Ala (A)  33	  4.4%
Arg (R)  32	  4.3%
Asn (N)  44	  5.9%
Asp (D)  39	  5.2%
Cys (C)  25	  3.3%
Gln (Q)  33	  4.4%
Glu (E)  56	  7.5%
Gly (G)  32	  4.3%
His (H)  20	  2.7%
Ile (I)  50	  6.7%
Leu (L)  66	  8.8%
Lys (K)  65	  8.7%
Met (M)  14	  1.9%
Phe (F)  40	  5.3%
Pro (P)  28	  3.7%
Ser (S)  54	  7.2%
Thr (T)  48	  6.4%
Trp (W)   9	  1.2%
Tyr (Y)  23	  3.1%
Val (V)  37	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3828
Hydrogen    H	      6001
Nitrogen    N	      1035
Oxygen      O	      1141
Sulfur      S	        39

Formula: C3828H6001N1035O1141S39
Total number of atoms: 12044

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    85270
Abs 0.1% (=1 g/l)   0.991, assuming all pairs of Cys residues form cystines


Ext. coefficient    83770
Abs 0.1% (=1 g/l)   0.974, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.86
This classifies the protein as unstable.



Aliphatic index: 79.24

Grand average of hydropathicity (GRAVY): -0.460