ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRHHDPNPF DEDEEIVNPF SKGGGRVPAA SRPVEYGQSL DATVDIPLDN MNDSSQKQRK 

        70         80         90        100        110        120 
LADWEAELRK KEMDIKRREE AIAKFGVQID DKNWPPFFPI IHHDIAKEIP VHAQKLQYLA 

       130        140        150        160        170        180 
FASWLGIVLC LVFNVIATMV CWIKGGGVKI FFLATIYALI GCPLSYVLWY RPLYRAMRTD 

       190        200        210        220        230        240 
SALKFGWFFF TYLIHIGFCI VAAIAPPIFF HGKSLTGVLA AIDVISDSLL AGIFYFIGFG 

       250        260 
LFCLESLLSL WVLQKIYLYF RGNK 

References and documentation are available.

Number of amino acids: 264

Molecular weight: 30060.17

Theoretical pI: 7.66

Amino acid composition: 
Ala (A)  22	  8.3%
Arg (R)  11	  4.2%
Asn (N)   8	  3.0%
Asp (D)  15	  5.7%
Cys (C)   5	  1.9%
Gln (Q)   7	  2.7%
Glu (E)  11	  4.2%
Gly (G)  18	  6.8%
His (H)   7	  2.7%
Ile (I)  25	  9.5%
Leu (L)  27	 10.2%
Lys (K)  16	  6.1%
Met (M)   5	  1.9%
Phe (F)  21	  8.0%
Pro (P)  14	  5.3%
Ser (S)  13	  4.9%
Thr (T)   6	  2.3%
Trp (W)   7	  2.7%
Tyr (Y)  10	  3.8%
Val (V)  16	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1409
Hydrogen    H	      2135
Nitrogen    N	       349
Oxygen      O	       361
Sulfur      S	        10

Formula: C1409H2135N349O361S10
Total number of atoms: 4264

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53650
Abs 0.1% (=1 g/l)   1.785, assuming all pairs of Cys residues form cystines


Ext. coefficient    53400
Abs 0.1% (=1 g/l)   1.776, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.06
This classifies the protein as unstable.



Aliphatic index: 102.73

Grand average of hydropathicity (GRAVY): 0.233