ProtParam
User-provided sequence:
10 20 30 40 50 60
MNRILSSASL LSNVSMPRQN KHKITKALCY AIIVASIGSI QFGYHLSELN APQQVLSCSE
70 80 90 100 110 120
FDIPMEGYPY DRTWLGKRGY KQCIPLNDEQ IGIVTSVFCI GGILGSYFAT SLANIYGRKF
130 140 150 160 170 180
SSLINCTLNI VGSLIIFNSN SYRGLIIGRI LVGISCGSLI VIIPLFIKEV APSGWEGLLG
190 200 210 220 230 240
SMTQICIRLG VLLTQGIALP LTDSYRWRWI LFGSFLIAVL NFFMWFIVDE SPKWLLAHGR
250 260 270 280 290 300
VTDAKLSLCK LRGVTFDEAA QEIQDWQLQI ESGDPLIEPT TTNSISGSNS LWKYLRDRTN
310 320 330 340 350 360
VKSRHVITVL LFGQQFCGIN SIVLYGTKII SQLYPQHAIR INFFISMVNV LVTILVSLLI
370 380 390 400 410 420
HSLPRKPLLM TSTVLVSVTA FIMGIAMNHN KMNLLIVFSF IYMGVFTMGL NPLPFIIMRE
430 440 450 460 470 480
VSKPQDMVLA QRYGTICNWV GTFIIAYTFP IIHDVLSGYV FIIFAIIACS ISAFIWKKVP
ETKRSG
References and
documentation are available.
Number of amino acids: 486
Molecular weight: 54331.28
Theoretical pI: 9.29
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2506
Hydrogen H 3953
Nitrogen N 631
Oxygen O 663
Sulfur S 25
Formula: C
2506H
3953N
631O
663S
25
Total number of atoms: 7778
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 79465
Abs 0.1% (=1 g/l) 1.463, assuming all pairs of Cys residues form cystines
Ext. coefficient 78840
Abs 0.1% (=1 g/l) 1.451, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.57
This classifies the protein as stable.
Aliphatic index: 118.11
Grand average of hydropathicity (GRAVY): 0.516