ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRQNGGGQR LRSARPTTIH DCALSGDLIA LQRLLKDNPS LLNERNPVMY HTPLHVSAGN 

        70         80         90        100        110        120 
GNVDIVKYLL AWTGSDKVEL EAMNTYGETP LHMAAKNGCN EAAKLLLESG AFIEAKASNG 

       130        140        150        160        170        180 
MTPLHLAVWY SITAKEISTV KTLLDHNADC SAKDNEGMTP LDHLPQGQGS EKLRELLRWF 

       190        200        210        220        230        240 
LQEQRKRSAL EQCGKTKAKM ELLEDELSNI VGLSELKTQL RKWAKGMLLD ERRRALGLNI 

       250        260        270        280        290        300 
GTRRPPHMAF LGNPGTGKTM VARVLGKLLN TVGILPTDKV TEVQRTDLVG EFVGHTGPKT 

       310        320        330        340        350        360 
RRKIQEAEGG ILFVDEAYRL IPMQKADDKD YGLEALEEIM SVMDTGKIVV IFAGYSEPMK 

       370        380        390        400        410        420 
RVIASNEGFC RRVTKFFNFS DFSAKELAQI LHIKMNNQGE DTLFYGFRLH ESCTLQEIAS 

       430        440        450        460        470        480 
LIEAETTEKQ RKEMNGGLVD TLLVNARENL DLRLSFECVD TEEICTIKLE DLEAGLRVFS 


Q 

References and documentation are available.

Number of amino acids: 481

Molecular weight: 53674.61

Theoretical pI: 6.19

Amino acid composition: 
Ala (A)  35	  7.3%
Arg (R)  29	  6.0%
Asn (N)  24	  5.0%
Asp (D)  24	  5.0%
Cys (C)   8	  1.7%
Gln (Q)  17	  3.5%
Glu (E)  42	  8.7%
Gly (G)  38	  7.9%
His (H)  11	  2.3%
Ile (I)  22	  4.6%
Leu (L)  62	 12.9%
Lys (K)  32	  6.7%
Met (M)  16	  3.3%
Phe (F)  15	  3.1%
Pro (P)  15	  3.1%
Ser (S)  23	  4.8%
Thr (T)  31	  6.4%
Trp (W)   4	  0.8%
Tyr (Y)   8	  1.7%
Val (V)  25	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2352
Hydrogen    H	      3811
Nitrogen    N	       667
Oxygen      O	       717
Sulfur      S	        24

Formula: C2352H3811N667O717S24
Total number of atoms: 7571

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34420
Abs 0.1% (=1 g/l)   0.641, assuming all pairs of Cys residues form cystines


Ext. coefficient    33920
Abs 0.1% (=1 g/l)   0.632, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.44
This classifies the protein as stable.



Aliphatic index: 90.46

Grand average of hydropathicity (GRAVY): -0.340