ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNRRLLVRSI SCFQPLSRIT FGRPNTPFLR KYADTSTAAN TNSTILRKQL LSLKPISASD 

        70         80         90        100        110        120 
SLFISCTVFN SKGNIISMSE KFPKWSFLTE HSLFPRDLRK IDNSSIDIIP TIMCKPNCIV 

       130        140        150        160        170        180 
INLLHIKALI ERDKVYVFDT TNPSAAAKLS VLMYDLESKL SSTKNNSQFY EHRALESIFI 

       190        200        210        220        230        240 
NVMSALETDF KLHSQICIQI LNDLENEVNR LKLRHLLIKS KDLTLFYQKT LLIRDLLDEL 

       250        260        270        280        290        300 
LENDDDLANM YLTVKKSPKD NFSDLEMLIE TYYTQCDEYV QQSESLIQDI KSTEEIVNII 

       310        320        330        340        350        360 
LDANRNSLML LELKVTIYTL GFTVASVLPA FYGMNLKNFI EESEWGFTSV AVFSIVSALY 

       370        380        390        400        410        420 
ITKKNFNSLR SVTKMTMYPN SPANSSVYPK TSASIALTNK LKRRRKWWKS TKQRLGVLLY 

       430        440        450        460        470 
GSSYTNKANL SNNKINKGFS KVKKFNMEND IKNKQNRDMI WKWLIEDKKN 

References and documentation are available.

Number of amino acids: 470

Molecular weight: 54202.81

Theoretical pI: 9.42

Amino acid composition: 
Ala (A)  20	  4.3%
Arg (R)  21	  4.5%
Asn (N)  39	  8.3%
Asp (D)  24	  5.1%
Cys (C)   6	  1.3%
Gln (Q)  12	  2.6%
Glu (E)  23	  4.9%
Gly (G)   8	  1.7%
His (H)   5	  1.1%
Ile (I)  38	  8.1%
Leu (L)  58	 12.3%
Lys (K)  44	  9.4%
Met (M)  13	  2.8%
Phe (F)  22	  4.7%
Pro (P)  14	  3.0%
Ser (S)  50	 10.6%
Thr (T)  30	  6.4%
Trp (W)   6	  1.3%
Tyr (Y)  16	  3.4%
Val (V)  21	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2435
Hydrogen    H	      3904
Nitrogen    N	       644
Oxygen      O	       712
Sulfur      S	        19

Formula: C2435H3904N644O712S19
Total number of atoms: 7714

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57215
Abs 0.1% (=1 g/l)   1.056, assuming all pairs of Cys residues form cystines


Ext. coefficient    56840
Abs 0.1% (=1 g/l)   1.049, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.72
This classifies the protein as unstable.



Aliphatic index: 96.87

Grand average of hydropathicity (GRAVY): -0.257