ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSEEQYYAA TQLYKDPCAF QRGPVPEFSA NPPACLYMGR QPPPPPPPQF TSSLGSLEQG 

        70         80         90        100        110        120 
SPPDISPYEV PPLASDDPAG AHLHHHLPAQ LGLAHPPPGP FPNGTEPGGL EEPNRVQLPF 

       130        140        150        160        170        180 
PWMKSTKAHA WKGQWAGGAY TAEPEENKRT RTAYTRAQLL ELEKEFLFNK YISRPRRVEL 

       190        200        210        220        230        240 
AVMLNLTERH IKIWFQNRRM KWKKEEDKKR SSGTPSGGGG GEEPEQDCAV TSGEELLAVP 

       250        260        270        280 
PLPPPGGAVP PGVPAAVREG LLPSGLSVSP QPSSIAPLRP QEPR 

References and documentation are available.

Number of amino acids: 284

Molecular weight: 30999.05

Theoretical pI: 6.40

Amino acid composition: 
Ala (A)  24	  8.5%
Arg (R)  16	  5.6%
Asn (N)   8	  2.8%
Asp (D)   6	  2.1%
Cys (C)   3	  1.1%
Gln (Q)  14	  4.9%
Glu (E)  25	  8.8%
Gly (G)  25	  8.8%
His (H)   7	  2.5%
Ile (I)   5	  1.8%
Leu (L)  25	  8.8%
Lys (K)  13	  4.6%
Met (M)   5	  1.8%
Phe (F)   8	  2.8%
Pro (P)  46	 16.2%
Ser (S)  19	  6.7%
Thr (T)  11	  3.9%
Trp (W)   5	  1.8%
Tyr (Y)   8	  2.8%
Val (V)  11	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1388
Hydrogen    H	      2136
Nitrogen    N	       386
Oxygen      O	       407
Sulfur      S	         8

Formula: C1388H2136N386O407S8
Total number of atoms: 4325

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39545
Abs 0.1% (=1 g/l)   1.276, assuming all pairs of Cys residues form cystines


Ext. coefficient    39420
Abs 0.1% (=1 g/l)   1.272, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 78.62
This classifies the protein as unstable.



Aliphatic index: 60.88

Grand average of hydropathicity (GRAVY): -0.724