ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSESREDMA INSIKLLAGN SHPDLAEQIS KKLGIPLSKV GVYQYSNKET SVTIGESLRD 

        70         80         90        100        110        120 
EDVYIIQTGI GEQEINDFLM ELLILIHACQ IASARKITTV IPNFPYARQD KKDKSRAPIT 

       130        140        150        160        170        180 
AKLVANLLQT AGADHVITMD LHASQIQGFF HIPVDNLYAE PSVLNYIRAR KTDFDNAILV 

       190        200        210        220        230        240 
SPDAGGAKRV AALADKLDLN FALIHKERQK ANEVSKMVLV GDVTNKSCLL VDDMADTCGT 

       250        260        270        280        290        300 
LVKACDTLME HGAKEVIAIV THGIFSGSAR EKLRNSRLSR IVCTNTVPVD LDLPIADQID 

       310        320 
ISPTFAEAIR RLHNGESVSY LFTHAPV 

References and documentation are available.

Number of amino acids: 327

Molecular weight: 35846.12

Theoretical pI: 5.94

Amino acid composition: 
Ala (A)  32	  9.8%
Arg (R)  15	  4.6%
Asn (N)  16	  4.9%
Asp (D)  24	  7.3%
Cys (C)   5	  1.5%
Gln (Q)  11	  3.4%
Glu (E)  17	  5.2%
Gly (G)  16	  4.9%
His (H)  10	  3.1%
Ile (I)  29	  8.9%
Leu (L)  32	  9.8%
Lys (K)  20	  6.1%
Met (M)   7	  2.1%
Phe (F)   9	  2.8%
Pro (P)  12	  3.7%
Ser (S)  23	  7.0%
Thr (T)  18	  5.5%
Trp (W)   0	  0.0%
Tyr (Y)   7	  2.1%
Val (V)  24	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1579
Hydrogen    H	      2567
Nitrogen    N	       439
Oxygen      O	       485
Sulfur      S	        12

Formula: C1579H2567N439O485S12
Total number of atoms: 5082

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10680
Abs 0.1% (=1 g/l)   0.298, assuming all pairs of Cys residues form cystines


Ext. coefficient    10430
Abs 0.1% (=1 g/l)   0.291, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.07
This classifies the protein as stable.



Aliphatic index: 103.82

Grand average of hydropathicity (GRAVY): -0.060