ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSFSAFSEM FGSDYEPQGG DYCPTLATSC PKKPAGRKKF RETRHPIYRG VRQRNSGKWV
70 80 90 100 110 120
SEVREPNKKT RIWLGTFQTA EMAARAHDVA ALALRGRSAC LNFADSAWRL RIPESTCAKD
130 140 150 160 170 180
IQKAAAEAAL AFQDETCDTT TTNHGLDMEE TMVEAIYTPE QSEGAFYMDE ETMFGMPTLL
190 200 210
DNMAEGMLLP PPSVQWNHNY DGEGDGDVSL WSY
References and
documentation are available.
Number of amino acids: 213
Molecular weight: 23829.63
Theoretical pI: 4.99
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1037
Hydrogen H 1585
Nitrogen N 289
Oxygen O 328
Sulfur S 15
Formula: C
1037H
1585N
289O
328S
15
Total number of atoms: 3254
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38180
Abs 0.1% (=1 g/l) 1.602, assuming all pairs of Cys residues form cystines
Ext. coefficient 37930
Abs 0.1% (=1 g/l) 1.592, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.00
This classifies the protein as unstable.
Aliphatic index: 53.29
Grand average of hydropathicity (GRAVY): -0.607