ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSGVRMVTR SRSRATRIAS EGCREELAPR EAAAEGRKSH RPVRHPRRTQ KTHVAYEAAN
70 80 90 100 110 120
GEEGEDAEPL KVPVWEPQNW QQQLANIRIM RSKKDAPVDQ LGAEHCYDAS APPKVRRYQV
130 140 150 160 170 180
LLSLMLSSQT KDQVTAGAMQ RLRARGLTVE SILQTDDDTL GRLIYPVGFW RNKVKYIKQT
190 200 210 220 230 240
TAILQQRYEG DIPASVAELV ALPGVGPKMA HLAMAVAWGT ISGIAVDTHV HRIANRLRWT
250 260 270 280 290 300
KKMTKTPEET RKNLEEWLPR VLWSEVNGLL VGFGQQICLP VHPRCQACLN KALCPAAQDL
References and
documentation are available.
Number of amino acids: 300
Molecular weight: 33646.73
Theoretical pI: 9.66
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1470
Hydrogen H 2388
Nitrogen N 450
Oxygen O 427
Sulfur S 14
Formula: C
1470H
2388N
450O
427S
14
Total number of atoms: 4749
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47815
Abs 0.1% (=1 g/l) 1.421, assuming all pairs of Cys residues form cystines
Ext. coefficient 47440
Abs 0.1% (=1 g/l) 1.410, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.99
This classifies the protein as unstable.
Aliphatic index: 83.60
Grand average of hydropathicity (GRAVY): -0.479