ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSILDRNVR SSETTLIKPE SEFDNWLSDE NDGASHINVN KDSSSVLSAS SSTWFEPLEN
70 80 90 100 110 120
IISSASSSSI GSPIEDQFIS SNNEESALFP TDQFFSNPSS YSHSPEVSSS IKREEDDNAL
130 140 150 160 170 180
SLADFEPASL QLMPNMINTD NNDDSTPLKN EIELNDSFIK TNLDAKETKK RAPRKRLTPF
190 200 210 220 230 240
QKQAHNKIEK RYRININTKI ARLQQIIPWV ASEQTAFEVG DSVKKQDEDG AETAATTPLP
250 260 270 280 290
SAAATSTKLN KSMILEKAVD YILYLQNNER LYEMEVQRLK SEIDTLKQDQ K
References and
documentation are available.
Number of amino acids: 291
Molecular weight: 32689.08
Theoretical pI: 4.75
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1416
Hydrogen H 2251
Nitrogen N 391
Oxygen O 486
Sulfur S 5
Formula: C
1416H
2251N
391O
486S
5
Total number of atoms: 4549
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 0.733
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 62.00
This classifies the protein as unstable.
Aliphatic index: 74.81
Grand average of hydropathicity (GRAVY): -0.755