ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSILDRNVR SSETTLIKPE SEFDNWLSDE NDGASHINVN KDSSSVLSAS SSTWFEPLEN 

        70         80         90        100        110        120 
IISSASSSSI GSPIEDQFIS SNNEESALFP TDQFFSNPSS YSHSPEVSSS IKREEDDNAL 

       130        140        150        160        170        180 
SLADFEPASL QLMPNMINTD NNDDSTPLKN EIELNDSFIK TNLDAKETKK RAPRKRLTPF 

       190        200        210        220        230        240 
QKQAHNKIEK RYRININTKI ARLQQIIPWV ASEQTAFEVG DSVKKQDEDG AETAATTPLP 

       250        260        270        280        290 
SAAATSTKLN KSMILEKAVD YILYLQNNER LYEMEVQRLK SEIDTLKQDQ K 

References and documentation are available.

Number of amino acids: 291

Molecular weight: 32689.08

Theoretical pI: 4.75

Amino acid composition: 
Ala (A)  20	  6.9%
Arg (R)  11	  3.8%
Asn (N)  24	  8.2%
Asp (D)  21	  7.2%
Cys (C)   0	  0.0%
Gln (Q)  13	  4.5%
Glu (E)  26	  8.9%
Gly (G)   4	  1.4%
His (H)   3	  1.0%
Ile (I)  21	  7.2%
Leu (L)  23	  7.9%
Lys (K)  21	  7.2%
Met (M)   5	  1.7%
Phe (F)  10	  3.4%
Pro (P)  14	  4.8%
Ser (S)  41	 14.1%
Thr (T)  17	  5.8%
Trp (W)   3	  1.0%
Tyr (Y)   5	  1.7%
Val (V)   9	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1416
Hydrogen    H	      2251
Nitrogen    N	       391
Oxygen      O	       486
Sulfur      S	         5

Formula: C1416H2251N391O486S5
Total number of atoms: 4549

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.733

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.00
This classifies the protein as unstable.



Aliphatic index: 74.81

Grand average of hydropathicity (GRAVY): -0.755