ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSILLNIKD NWDQNANVYK KEEHGKWSIT VPAKEDGSCP IPHNSVIKIA VSRHGATHFK 

        70         80         90        100        110        120 
LSPWATFVTC PNPKETVIYH QNFWNPPEKY QLKEARPARP ASLRIYEAHV GISSSEGKIN 

       130        140        150        160        170        180 
TYREFADDVL PRIQKQGYNA IQLMAVMEHV YYASFGYQVS NFFAVSSRCG TPEDLKYLVD 

       190        200        210        220        230        240 
KAHSLGIFML LDVVHSHASK NVEDGLNQWD GSNGGYFHDN ARGYHNLWDS RLFDYTQTET 

       250        260        270        280        290        300 
LRFLLSNVRW WVEEYGFDGF RFDGVSSMIY HSHGMNDDFC GGYPMYFGLN ADTDSLVYLM 

       310        320        330        340        350        360 
LANDFLHKKY PFMITIAEEV SGMPGICRPV EEGGQGFDYR LAMALPDMWI KILKHTSDED 

       370        380        390        400        410        420 
WKIDDIVFNL ENRRYAEKHV AYAESHDQAL VGDKTIAFWL MDKEMYDFMS TDSPLTPIID 

       430        440        450        460        470        480 
RGLSLHKLIR LITIGLGGEA WLNFIGNEFG HPEWLDFPRV GNGESFHYAR RQFNLVDAEY 

       490        500        510        520        530        540 
LRYKFLNNWD REMMLLEERT GFLHKGYAYT SWKHDGDKTI VFERGGLVFV INLHPTKSFA 

       550        560        570        580        590        600 
DYSIGVNTPG RYRIALNSDE SKFGGHNRID NSIKFHTTDD GYAGRRHRLQ VYITCRTAIV 


LEKEDD 

References and documentation are available.

Number of amino acids: 606

Molecular weight: 69918.84

Theoretical pI: 5.93

Amino acid composition: 
Ala (A)  35	  5.8%
Arg (R)  32	  5.3%
Asn (N)  34	  5.6%
Asp (D)  44	  7.3%
Cys (C)   6	  1.0%
Gln (Q)  13	  2.1%
Glu (E)  36	  5.9%
Gly (G)  46	  7.6%
His (H)  27	  4.5%
Ile (I)  36	  5.9%
Leu (L)  48	  7.9%
Lys (K)  32	  5.3%
Met (M)  17	  2.8%
Phe (F)  34	  5.6%
Pro (P)  23	  3.8%
Ser (S)  37	  6.1%
Thr (T)  26	  4.3%
Trp (W)  15	  2.5%
Tyr (Y)  32	  5.3%
Val (V)  33	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      3161
Hydrogen    H	      4728
Nitrogen    N	       850
Oxygen      O	       909
Sulfur      S	        23

Formula: C3161H4728N850O909S23
Total number of atoms: 9671

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   130555
Abs 0.1% (=1 g/l)   1.867, assuming all pairs of Cys residues form cystines


Ext. coefficient   130180
Abs 0.1% (=1 g/l)   1.862, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.36
This classifies the protein as stable.



Aliphatic index: 75.63

Grand average of hydropathicity (GRAVY): -0.444