ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSLIFGKQL AFHKIVPTTA IGWLVPLGNP SLQIPGQKQL GSIHRWLREK LQQDHKDTED 

        70         80         90        100        110        120 
KDFFSNNGIL LAVPKKKVSH QKKRQKLYGP GKKQLKMIHH LNKCPSCGHY KRANTLCMYC 

       130        140        150        160        170        180 
VGQISHIWKT HTAKEEIKPR QEEELSELDQ RVLYPGRRDT KYTKDLKDKD NYLERRVRTL 


KKD 

References and documentation are available.

Number of amino acids: 183

Molecular weight: 21436.93

Theoretical pI: 9.86

Amino acid composition: 
Ala (A)   5	  2.7%
Arg (R)  11	  6.0%
Asn (N)   7	  3.8%
Asp (D)  10	  5.5%
Cys (C)   4	  2.2%
Gln (Q)  12	  6.6%
Glu (E)   9	  4.9%
Gly (G)  11	  6.0%
His (H)   9	  4.9%
Ile (I)  10	  5.5%
Leu (L)  20	 10.9%
Lys (K)  26	 14.2%
Met (M)   3	  1.6%
Phe (F)   4	  2.2%
Pro (P)   9	  4.9%
Ser (S)   8	  4.4%
Thr (T)   9	  4.9%
Trp (W)   3	  1.6%
Tyr (Y)   6	  3.3%
Val (V)   7	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	       956
Hydrogen    H	      1544
Nitrogen    N	       282
Oxygen      O	       264
Sulfur      S	         7

Formula: C956H1544N282O264S7
Total number of atoms: 3053

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25690
Abs 0.1% (=1 g/l)   1.198, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   1.187, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.29
This classifies the protein as unstable.



Aliphatic index: 77.76

Grand average of hydropathicity (GRAVY): -0.920