ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSLYELDPK WKKLLKTDNF LGGLTVNEFV QELSKDHRND VLIDANTKNL PTNEKDQDAI 

        70         80         90        100        110        120 
REAIWKQLDP KPYIRTFEST LKELKNLNEE TLNKRQYFSE QVATQEVIHS ENVIKLSKDL 

       130        140        150        160        170        180 
HTTLLTFDKL DDRLTNVTQV VSPLGDKLET AIKKKQNYIQ SVELIRRYND FYSMGKSDIV 

       190        200        210        220        230        240 
EQLRLSKNWK LNLKSVKLMK NLLILSSKLE TSSIPKTINT KLVIEKYSEM MENELLENFN 

       250        260        270        280        290        300 
SAYRENNFTK LNEIAIILNN FNGGVNVIQS FINQHDYFID TKQIDLENEF ENVFIKNVKF 

       310        320        330        340        350        360 
KEQLIDFENH SVIIETSMQN LINDVETVIK NESKIVKRVF EEKATHVIQL FIQRVFAQKI 

       370        380        390        400        410        420 
EPRFEVLLRN SLSISNLAYV RILHGLFTLF GKFTKSLIDY FQLLEIDDSN QILSTTLEQC 

       430        440        450        460        470        480 
FADLFSHYLY DRSKYFGIEK RSLEAILVDM TSKFTVNYDK EINKRVLLDK YKEKLSTNVD 

       490        500        510        520        530        540 
AFMHSPRGNT HSRQDSTSRS KLSQFNSFLK THLDKDHLSL NRTNTLSDSF NNSSSSTQYD 

       550        560        570        580        590        600 
VANNSSSLVN SSFTASDIDN SPNSPANYSL NDVDSMLKCV VESTARVMEL IPNKAHLYIL 

       610        620        630        640        650        660 
EILKIMFLGI VDSYMEIALE VAYWKICKVD INKTAGVVNL NFLKFISMST EILDLLSISI 

       670        680        690        700        710        720 
KSIFLPLLNN SPEIKAQIIE MTNSQIQKME ILINIILQET ITVISTKFSA ILCKQKKKDF 

       730        740        750        760        770        780 
VPKSQELLDQ DTLPAIEIVN ILNLIFEQSS KFLKGKNLQT FLTLIGEELY GLLLSHYSHF 

       790        800        810        820        830        840 
QVNSIGGVVV TKDIIGYQTA IEDWGVASLI DKFATLRELA NLFTVQPELL ESLTKEGHLA 

       850        860        870 
DIGRDIIQSY ISNREDFNHD NFINSVKLNF R 

References and documentation are available.

Number of amino acids: 871

Molecular weight: 100342.00

Theoretical pI: 5.90

Amino acid composition: 
Ala (A)  29	  3.3%
Arg (R)  26	  3.0%
Asn (N)  73	  8.4%
Asp (D)  51	  5.9%
Cys (C)   4	  0.5%
Gln (Q)  37	  4.2%
Glu (E)  62	  7.1%
Gly (G)  21	  2.4%
His (H)  17	  2.0%
Ile (I)  79	  9.1%
Leu (L) 108	 12.4%
Lys (K)  75	  8.6%
Met (M)  15	  1.7%
Phe (F)  47	  5.4%
Pro (P)  16	  1.8%
Ser (S)  79	  9.1%
Thr (T)  51	  5.9%
Trp (W)   5	  0.6%
Tyr (Y)  25	  2.9%
Val (V)  51	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      4516
Hydrogen    H	      7197
Nitrogen    N	      1173
Oxygen      O	      1363
Sulfur      S	        19

Formula: C4516H7197N1173O1363S19
Total number of atoms: 14268

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65000
Abs 0.1% (=1 g/l)   0.648, assuming all pairs of Cys residues form cystines


Ext. coefficient    64750
Abs 0.1% (=1 g/l)   0.645, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.43
This classifies the protein as stable.



Aliphatic index: 104.04

Grand average of hydropathicity (GRAVY): -0.243