ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSMADTDRV NLTPIQRASE KSVQYHLKQV IGRGSYGVVY KAINKHTDQV VAIKEVVYEN 

        70         80         90        100        110        120 
DEELNDIMAE ISLLKNLNHN NIVKYHGFIR KSYELYILLE YCANGSLRRL ISRSSTGLSE 

       130        140        150        160        170        180 
NESKTYVTQT LLGLKYLHGE GVIHRDIKAA NILLSADNTV KLADFGVSTI VNSSALTLAG 

       190        200        210        220        230        240 
TLNWMAPEIL GNRGASTLSD IWSLGATVVE MLTKNPPYHN LTDANIYYAV ENDTYYPPSS 

       250        260        270        280        290        300 
FSEPLKDFLS KCFVKNMYKR PTADQLLKHV WINSTENVKV DKLNKFKEDF TDADYHWDAD 

       310        320        330        340        350        360 
FQEEKLNISP SKFSLAAAPA AWAENNQELD LMPPTESQLL SQLKSSSKPL TDLHVLFSVC 

       370        380        390        400        410        420 
SLENIADTII ECLSRTTVDK RLITAFGSIF VYDTQHNHSR LRLKFIAMGG IPLIIKFEHL 

       430        440        450        460        470        480 
AKEFVIDYPQ TLIECGIMYP PNFASLKTPK YILELVYRFY DLTSTAFWCR WCFKHLDISL 

       490        500        510        520        530        540 
LLNNIHERRA QSILLKLSSY APWSFEKILP SLIDSKLKKK ILISPQITYV VFKSINYMIT 

       550        560        570        580        590        600 
TNDDKIHKSA IPSSSSLPLS SSPTRNSPVN SVQSPSRSPV HSLMATRPSS PMRHKSISNF 

       610        620        630        640        650        660 
PHLTISSKSR LLIELPEGFF TWLTSFFVDM AQIKDLSVLK YFTKLCYLTV HINSTFLNDL 

       670        680        690        700        710        720 
LDNDAFFAFI RNIDTIIPFI DDAKTAAFIW KQITAICVEM SLDMDQMSAS LFSTAMNFIR 

       730        740        750        760        770        780 
KKNNTSISGL EIILNCLHFT LRNVNDDVAP TVGSSESHSV FLIKVNNDAA IELPIDQLVD 

       790        800        810        820        830        840 
LFYALNDDDV NLSKLISIFT KICSLPGFEN LTINIIFHPN FYEKIVSFFD TYFNSLLIQI 

       850        860        870        880        890        900 
DLLKFIKLIF SKSLLKLYDY TGQPDPIKQT EPNRRNKATV FKLRAILVQI TEFLNNNWNK 

       910        920        930        940        950        960 
DVPKRNSNQV GGDSVLICQL CEDIRSLSKK GSLQKVSSVT AAIGSSPTKD ERSNLRSSKD 

       970 
KSDGFSVPIT TFQT 

References and documentation are available.

Number of amino acids: 974

Molecular weight: 110241.72

Theoretical pI: 8.02

Amino acid composition: 
Ala (A)  53	  5.4%
Arg (R)  33	  3.4%
Asn (N)  64	  6.6%
Asp (D)  58	  6.0%
Cys (C)  13	  1.3%
Gln (Q)  27	  2.8%
Glu (E)  42	  4.3%
Gly (G)  28	  2.9%
His (H)  23	  2.4%
Ile (I)  82	  8.4%
Leu (L) 111	 11.4%
Lys (K)  70	  7.2%
Met (M)  17	  1.7%
Phe (F)  51	  5.2%
Pro (P)  42	  4.3%
Ser (S) 103	 10.6%
Thr (T)  60	  6.2%
Trp (W)  11	  1.1%
Tyr (Y)  33	  3.4%
Val (V)  53	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 103

Atomic composition:

Carbon      C	      4987
Hydrogen    H	      7845
Nitrogen    N	      1291
Oxygen      O	      1462
Sulfur      S	        30

Formula: C4987H7845N1291O1462S30
Total number of atoms: 15615

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   110420
Abs 0.1% (=1 g/l)   1.002, assuming all pairs of Cys residues form cystines


Ext. coefficient   109670
Abs 0.1% (=1 g/l)   0.995, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.53
This classifies the protein as unstable.



Aliphatic index: 98.50

Grand average of hydropathicity (GRAVY): -0.106