ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNSNELDLRN KYFYEIFGKK RKSDTSTPTQ LFSGSKVQTN INEISITNDE DEDSTEDENK 

        70         80         90        100        110        120 
ASLKDYTLGH DTGARYRIAP DCSSHQLKAS PVLHISTNLN SSPQSFTGDQ ISPTNKKISI 

       130        140        150        160        170        180 
NDSTRQDKGN SCTTTSSPSQ KRSNVLLPHV RKHSSPSLLS FSKNSGSHMG DPNQLSTPPT 

       190        200        210        220        230        240 
PKSAGHTMEL HSSFNGKHSS SSTSSLFALE SLKTQNRRSS NSSNHSSQYR RHTNQHQRHH 

       250        260        270        280        290        300 
SRSKSSPVSL TEISMIKGTP LVYPALLSLI AIKFKQTIKL STHKKMGLLY RDSFTGKQAI 

       310        320        330        340        350        360 
DTLCLIIGSL DRNLGMLIGK SLEAQKLFHD VLYDHGVRDS VLEIYELSSE SIFMAHQSQS 

       370        380        390        400        410        420 
STSIANTFSS SSSSVNSLRT KTEIYGVFVP LTHCYSSTCS LEKLCYSISC PNRLQQQANL 

       430        440        450        460        470        480 
HLKLGGGLKR NISLALDKED DERISWTNSV PKSVWESLSK QQIKRQEAIY ELFTTEKKFV 

       490        500        510        520        530        540 
KSLEIIRDTF MKKLLETNII PSDVRINFVK HVFAHINEIY SVNREFLKAL AQRQSLSPIC 

       550        560        570        580        590        600 
PGIADIFLQY LPFFDPFLSY IASRPYAKYL IETQRSVNPN FARFDDEVSN SSLRHGIDSF 

       610        620        630        640        650        660 
LSQGVSRPGR YSLLVREIIH FSDPVTDKDD LQMLMKVQDL LKDLMKRIDR ASGAAQDRYD 

       670        680        690        700        710        720 
VKVLKQKILF KNEYVNLGLN NEKRKIKHEG LLSRKDVNKT DASFSGDIQF YLLDNMLLFL 

       730        740        750        760        770        780 
KSKAVNKWHQ HTVFQRPIPL PLLFICPAED MPPIKRYVTE NPNCSAGVLL PQYQTSNPKN 

       790        800        810        820        830        840 
AIVFAYYGTK QQYQVTLYAP QPAGLQTLIE KVKQEQKRLL DETKHITFKQ MVGQFFHSYI 

       850        860        870        880        890        900 
NTNRVNDVLI CHAGKILLVA TNMGLFVLNY ATSINQKPVH LLHKISISQI SVLEEYKVMI 

       910        920        930        940        950        960 
LLIDKKLYGC PLDVIDDAEN ADFLFRKNSK VLFKYVAMFK DGFCNGKRII MIAHHFLHAA 

       970        980        990       1000       1010       1020 
QLLIVNPLIF DFNSGNFKKN LKAGLVDFSV DSEPLSFSFL ENKICIGCKK NIKILNVPEV 

      1030       1040       1050       1060       1070       1080 
CDKNGFKMRE LLNLHDNKVL ANMYKETFKV VSMFPIKNST FACFPELCFF LNKQGKREET 

      1090       1100       1110       1120       1130       1140 
KGCFHWEGEP EQFACSYPYI VAINSNFIEI RHIENGELVR CVLGNKIRML KSYAKKILYC 

      1150 
YEDPQGFEII ELLNF 

References and documentation are available.

Number of amino acids: 1155

Molecular weight: 131391.78

Theoretical pI: 9.17

Amino acid composition: 
Ala (A)  48	  4.2%
Arg (R)  48	  4.2%
Asn (N)  72	  6.2%
Asp (D)  57	  4.9%
Cys (C)  22	  1.9%
Gln (Q)  52	  4.5%
Glu (E)  57	  4.9%
Gly (G)  48	  4.2%
His (H)  38	  3.3%
Ile (I)  81	  7.0%
Leu (L) 123	 10.6%
Lys (K)  98	  8.5%
Met (M)  22	  1.9%
Phe (F)  62	  5.4%
Pro (P)  48	  4.2%
Ser (S) 123	 10.6%
Thr (T)  55	  4.8%
Trp (W)   4	  0.3%
Tyr (Y)  39	  3.4%
Val (V)  58	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 114
Total number of positively charged residues (Arg + Lys): 146

Atomic composition:

Carbon      C	      5877
Hydrogen    H	      9295
Nitrogen    N	      1601
Oxygen      O	      1725
Sulfur      S	        44

Formula: C5877H9295N1601O1725S44
Total number of atoms: 18542

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81485
Abs 0.1% (=1 g/l)   0.620, assuming all pairs of Cys residues form cystines


Ext. coefficient    80110
Abs 0.1% (=1 g/l)   0.610, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.87
This classifies the protein as unstable.



Aliphatic index: 87.60

Grand average of hydropathicity (GRAVY): -0.353