ProtParam
User-provided sequence:
10 20 30 40 50 60
MNSNGPLMEH GKRRVAYYYD SNIGNYYYGQ GHVMKPHRIR MTHHLVLNYG LYRNLEIFRP
70 80 90 100 110 120
FPASFEDMTR FHSDEYMTFL KSANPDNLKS FNKQMLKFNV GEDCPLFDGL YEFCQLSSGG
130 140 150 160 170 180
SLAAATKLNK QKVDIAINWM GGLHHAKKSE ASGFCYTNDI VLGILELLKY HKRVLYVDID
190 200 210 220 230 240
VHHGDGVEEA FYTTDRVMTV SFHKYGDFFP GTGDLKDIGA GKGKLYSVNV PLRDGITDVS
250 260 270 280 290 300
YQSIFKPIMT KVMERFDPCA VVLQCGADSL NGDRLGPFNL TLKGHGECAR FFRSYNVPLM
310 320 330 340 350 360
MVGGGGYTPR NVARCWTYET SIAVDKEVPN ELPYNDYFEY FGPNYRLHIE SSNAANENSS
370 380 390 400 410 420
DMLAKLQTDV IANLEQLTFV PSVQMRPIPE DALSALNDDS LIADQANPDK RLPPQITDGM
430 440 450 460
IQDDGDFYDG EREGDDRRNE SDAKRAAQFE SEGGEKRQKT E
References and
documentation are available.
Number of amino acids: 461
Molecular weight: 52137.63
Theoretical pI: 5.50
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2311
Hydrogen H 3529
Nitrogen N 631
Oxygen O 703
Sulfur S 23
Formula: C
2311H
3529N
631O
703S
23
Total number of atoms: 7197
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48625
Abs 0.1% (=1 g/l) 0.933, assuming all pairs of Cys residues form cystines
Ext. coefficient 48250
Abs 0.1% (=1 g/l) 0.925, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.20
This classifies the protein as stable.
Aliphatic index: 70.43
Grand average of hydropathicity (GRAVY): -0.526